03353575910

DIG Oligonucleotide 3′-End Labeling Kit, 2^nd generation

sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide), storage condition avoid repeated freeze/thaw cycles

• UNSPSC Code: 41105500
• NACRES: NA.55

Properties

• usage: sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide)
• Quality Level: 100
• manufacturer/tradename: Roche
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• greener alternative category: , Aligned
• shipped in: dry ice

Description

General description

The DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of single digoxigenin-labeled dideoxyuridine triphosphates (ddUTP) to the 3′-OH end of oligonucleotides. Thus, helps in labeling oligonucleotides.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation is for 3′-end labeling of oligonucleotides from 14 to 100 nucleotides in length with DIG-11-ddUTP and recombinant terminal transferase.
DIG-labeled oligonucleotides has been used in a variety of hybridization techniques:

• dot/slot blots
• colony/ plaque hybridizations
• Southern blots/ northern blots
• in situ hybridizations

Features and Benefits

• Fast hybridization kinetics, due to the small size of oligonucleotides
• Single-stranded probes, no renaturation during hybridization
• Sequence can be designed according to the experiment
• Specially suited for in situ hybridization; due to their small size, the oligonucleotides readily diffuse into fixed tissues and cells

Packaging

1 kit containing 9 components

Quality

Function tested in a dot blot.

Principle

One DIG-ddUTP molecule is added to the 3′-end of oligonucleotides by recombinant Terminal Transferase. This guarantees a very specific and distinct hybridization signal, which is detected by an enzyme-linked immunoassay with anti-DIG-AP antibody conjugate, and a color or chemiluminescence reaction.

Preparation Note

Activator: sodium sulfate, Tris
Working concentration: Oligonucleotides: 100 pmol
Up to 100 pmol (1 μg of a 30-mer) oligonucleotide can be labeled in a single standard labeling reaction.

Assay Time: The complete procedure from labeling the oligonucleotide to hybridization and detection of the first visible signal can be accomplished within less than 24 hours.

Sample Materials
Oligonucleotides of a length from 14 to 100 nucleotides, purified by HPLC or gel electrophoresis

Storage and Stability

Store at -15–-25 °C. (unopened kit)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Reaction Buffer 5x concentrated
• CoCl₂ Solution 25 mM
• DIG-ddUTP Solution 1 mM
• Recombinant Terminal Transferase 400 U/μl
• Control Oligonucleotide, unlabeled 20 pmol/μl
• Oligonucleotide, DIG-ddUTP labeled 2.5 pmol/μl
• Control DNA, 2.5 pmol/μl pUC 18 DNA, supercoiled
Glycogen Solution 20 mg/ml
DNA Dilution Buffer, 50 μg/ml fish sperm DNA

See All (9)

Safety Information

• Pictograms: GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332 - H350i - H360F - H411
• Precautionary Statements: P201 - P261 - P273 - P280 - P308 + P313 - P391
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): does not flash
• Flash Point(C): does not flash