SCT249
BioTracker™ Spermidine Polyamine Green Fluorescent Live Cell Probe
Human
Synonym(s):
BioTracker™ Spermidine Polyamine Green Fluorescent Live Cell Probe, clickable polyamine probe, live cell probe, polyamine probe, polyamine transport system probe, spermidine probe
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All Photos(2)
About This Item
UNSPSC Code:
41106514
NACRES:
NB.12
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product name
BioTracker™ Spermidine Polyamine Green Fluorescent Live Cell Probe,
biological source
human
Quality Level
packaging
vial of 500 μg
manufacturer/tradename
Millipore
technique(s)
cell culture | stem cell: suitable
shipped in
dry ice
storage temp.
−20°C
Application
The relationship between polyamine systems and cancer---as well as critical normal cell processes--- led to the creation of clickable polyamine derivatives which are innovative and novel tools for functional studies of the polyamine transport system (PTS). Spermidine and other important polyamine derivatives were conjugated to a BODIPY fluorophore through click chemistry.
MW: 599.44 g/mol
SPECTRAL PROPERTIES
λex: 494 nm
λem: 508 nm
MW: 599.44 g/mol
SPECTRAL PROPERTIES
λex: 494 nm
λem: 508 nm
Features and Benefits
The BioTracker Clickable Polyamine Spermidine Live Cell Probe is click-conjugated to a green fluorophore to enable the study of polyamine tracking with superior cellular uptake. Probe may be dissolved in DMSO to make a 10mM stock solution. 1 uL of a 10 mM stock solution may be added to 1 mL media to produce a 10 uM working solution. Add probe/media solution to washed cells and incubate at 37C for up to 4h.
Target description
Polyamines play a variety of critical roles in the cell. Some of these processes include gene expression, cell proliferation and differentiation, and cellular stress management.
Polyamine dysregulation is key in a variety of cancers. In colorectal cancer, polyamine content and biosynthesis is significantly higher when compared to normal colorectal tissue. A relationship between polyamines and their metabolites has been used to observe progression in cancers of the breast, lung, colorectal system, ovary, prostate, and pancreas.
In normal cells, there is tight regulation of polyamines through normal cellular mechanisms. One of these is the polyamine transport system (PTS). The characteristics of the PTS are not fully understood, but study of the PTS has shown that many cancer cells have high PTS activity, suggesting this system as a promising target for cancer research.
Polyamine dysregulation is key in a variety of cancers. In colorectal cancer, polyamine content and biosynthesis is significantly higher when compared to normal colorectal tissue. A relationship between polyamines and their metabolites has been used to observe progression in cancers of the breast, lung, colorectal system, ovary, prostate, and pancreas.
In normal cells, there is tight regulation of polyamines through normal cellular mechanisms. One of these is the polyamine transport system (PTS). The characteristics of the PTS are not fully understood, but study of the PTS has shown that many cancer cells have high PTS activity, suggesting this system as a promising target for cancer research.
Storage and Stability
Store at -20 °C, protected from light
Other Notes
This spermidine derivative click-conjugated to BODIPY is optimized for the study of the polyamine transport system, which is exploited in diverse cancers.
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Roeland Vanhoutte et al.
Chembiochem : a European journal of chemical biology, 19(9), 907-911 (2018-02-17)
Polyamines are essential for cell growth and differentiation, but their trafficking by the polyamine transport system is not fully understood. Herein, the synthesis of several azido-derivatized polyamines for easy conjugation by click chemistry is described. Attachment of a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)
Norin Nabil Hamouda et al.
The Journal of biological chemistry, 296, 100182-100182 (2020-12-15)
Polyamines, such as putrescine, spermidine, and spermine, are physiologically important polycations, but the transporters responsible for their uptake in mammalian cells remain poorly characterized. Here, we reveal a new component of the mammalian polyamine transport system using CHO-MG cells, a
Sarah van Veen et al.
Nature, 578(7795), 419-424 (2020-01-31)
ATP13A2 (PARK9) is a late endolysosomal transporter that is genetically implicated in a spectrum of neurodegenerative disorders, including Kufor-Rakeb syndrome-a parkinsonism with dementia1-and early-onset Parkinson's disease2. ATP13A2 offers protection against genetic and environmental risk factors of Parkinson's disease, whereas loss
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