The information provided by the supplier for SCT117 indicates that the product is at a 200x concentration. To perform staining of fixed and permeabilized cells for cell number normalization, it is recommended to conduct a standard curve of cell density and test RedDot™1 at 1X and 2X final concentration in order to determine the optimal staining concentration for achieving a linear fluorescence signal for your specific cell type.
SCT117
BioTracker NIR694 Nuclear Dye (Water)
Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Synonym(s):
Live cell imaging probe
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352207
NACRES:
NA.47
Pricing and availability is not currently available.
Recommended Products
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
General description
The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).
Spectral Properties
Absorbance: 662nm
Emission: 694nm
The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).
Spectral Properties
Absorbance: 662nm
Emission: 694nm
Application
Live cell fluorescent imaging
Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Quality
Spectral Properties
Absorbance: 662nm
Emission: 694nm
Absorbance: 662nm
Emission: 694nm
Physical form
Liquid
Storage and Stability
Store BioTracker NIR694 Nuclear Dye (Water) at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
The data sheet indicates to dilute BioTracker™ NIR694 Nuclear Dye in cell culture medium or PBS to a final concentration of 1X. However, it does not specify the starting concentration, leaving uncertainty about the appropriate dilution ratio. Can you provide guidance on the starting concentration and the amount needed for dilution?
1 answer-
Helpful?
-
-
What is the stock concentration of this product? We purchased it recently, but it doesn’t say on the website or the product documentation.
1 answer-
SCT117 is provided at a 200X concentration.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
