The height of the feet or distance between the membrane and bottom of the well ranges from 1 - 2 mm. Please see the image below:
PIHP01250
Millicell® Standing Cell Culture Inserts
pore size 0.4 μm, diam. 12 mm, translucent polycarbonate membrane, Tissue Culture (TC)-treated surface, size 24 wells, sterile
Synonym(s):
Millicell Cell Culture Insert, 12 mm, polycarbonate, 0.4 µm, Millicell-PCF, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate
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About This Item
UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22
material:
polystyrene housing
translucent polycarbonate membrane
translucent polycarbonate membrane
pore size:
0.4 μm
sterility:
γ-irradiated
sterile
sterile
binding type:
Tissue Culture (TC)-treated surface
Pricing and availability is not currently available.
Recommended Products
material
polystyrene housing
translucent polycarbonate membrane
Quality Level
sterility
γ-irradiated
sterile
feature
hydrophilic
100x106 pores/cm2 (pore density)
packaging
pack of 50
manufacturer/tradename
Millicell®
parameter
50 °C max. temp.
technique(s)
cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable
H
10.5 mm
diam.
12 mm
size
24 wells
surface area
0.6 cm2
working volume
0.6 mL
color
translucent membrane
matrix
Isopore™
pore size
0.4 μm
binding type
Tissue Culture (TC)-treated surface
shipped in
ambient
General description
Device Configuration: 24-well plate, 0.4μm pore size
With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.
Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies
Membrane Type:
Isopore Membrane (polycarbonate)
- For growth of attachment-dependent cells without matrix
Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry
With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.
Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies
Membrane Type:
Isopore Membrane (polycarbonate)
- For growth of attachment-dependent cells without matrix
Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry
Legal Information
ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.
Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.
Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.
Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.
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What is the height of the "feet" of the PIHP01250 Millicell Cell Culture Inserts? Meaning, how much clearance is there between the bottom of the well and the bottom of the membrane?
1 answer -
Regarding the PIHP01250 & PTHT12H48 membrane insert products: what is the thickness of the membrane? Is the bottom totally flat, i.e. if I placed it down on a flat surface, would the entire area of the membrane be in direct contact with the surface?
1 answer-
The thickness of the polycarbonate and PET membranes range from 7-20 um. The standing inserts (PIHP01250) have small "feet" that contact the bottom of the wells. The hanging inserts (PTHT12H48) are suspended by 'wings' that rest on the sides of the wells. Please see the image below:
Helpful?
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What is the thickness of the membrane in these inserts? We need this information to evaluate if it's possible to image through the membrane with a confocal microscope.
1 answer-
The thickness specification for the PIHP01250 Millicell Cell Culture Insert, which is made of polycarbonate and has a pore size of 0.4 µm, ranges between 9-11 microns.
Helpful?
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