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MPSB-10ML

Millipore

mPAGE® 4X LDS Sample Buffer

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.56
Pricing and availability is not currently available.

form

liquid

packaging

vial of 10 mL

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General description

Used to prepare protein samples for denaturing polyacrylamide gel electrophoresis (PAGE) with mPAGE and most other Bis-Tris gels. LDS sample buffer contains lithium dodecyl sulfate with pH at 8.4 for optimal activity of the reducing agent.

  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Application

Western Blotting

Legal Information

MPAGE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H315,H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–2 of 2 Questions  

  1. Can Laemmli buffer be used instead of the sample buffer?

    1 answer

    1. Laemmli buffer is commonly used with Tris-Glycine gels, while mPAGE gels use a different chemistry (Bis-Tris) and require LDS buffer. Therefore, it is recommended to use the LDS buffer, which can be prepared using the formula provided in the mPAGE user guide.

      Helpful?

  2. Est ce que ce tampon contient déjà un agent réducteur tel que le DTT ou le béta mercapto ethanol ?

    1 answer

    1. This product requires the addition of a reducing agent. Either Dithiothrietol (DDT) or beta-mercaptoethanol (BME) may be used. The recommended final concentration is 100 mM DTT or 25 mM BME. Please see the link below to access the mPAGE™ Bis-Tris Precast SDS-PAGE Gels User Guide, page 7:
      https://www.emdmillipore.com/US/en/product/mPAGE-8-160-0-Bis-Tris-Precast-Gel-10x8-cm-12-well,MM_NF-MP81G12#documentation

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