To reduce the occurrence of sample-related bead aggregation, the following steps can be taken:
•Spin samples at 10,000 x g for 10 minutes immediately before preparing sample dilutions or loading samples into the plate.
•Resuspend the plate in Wash Buffer instead of Sheath or Drive Fluid. The plate must be read within a couple of hours, and the buffer should be switched to Sheath or Drive Fluid for longer storage.
•Dilute samples at a ratio of 1:2 or 1:4.
•Perform more wash cycles after the primary incubation step of the assay to remove substances that cause bead aggregation.
MCYTOMAG-70K-PMX
MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex - Immunology Multiplex Assay
Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Synonym(s):
Luminex® Mouse cytokine immunoassay, Millipore Mouse cytokine immunoassay, Mouse cytokine Multiplex Immunoassay
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Pricing and availability is not currently available.
Recommended Products
Quality Level
species reactivity
mouse
manufacturer/tradename
Milliplex®
assay range
accuracy: 85-107%
standard curve range: 3.2-10,000 pg/mL
technique(s)
multiplexing: suitable
compatibility
configured for Premixed
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
MILLIPLEX® Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Panel Type: Cytokines/Chemokines
- More flexible plate and plate washer options
- Improved performance with turbid serum/plasma samples
- Assay results equivalent to non- beads
- Automated washing avoids many problems associated with vacuum filtration washing
MILLIPLEX® Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Panel Type: Cytokines/Chemokines
Application
- Analytes: G-CSF, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, MCP-1, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α
Other Notes
Sensitivity: Refer to kit protocol for sensitivities for individual cytokines.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
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Dana M Hardbower et al.
Amino acids, 48(10), 2375-2388 (2016-04-15)
We reported that arginase 2 (ARG2) deletion results in increased gastritis and decreased bacterial burden during Helicobacter pylori infection in mice. Our studies implicated a potential role for inducible nitric oxide (NO) synthase (NOS2), as Arg2 (-/-) mice exhibited increased
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American journal of physiology. Gastrointestinal and liver physiology, 305(3), G225-G240 (2013-05-25)
L-Arginine (L-Arg) is a semiessential amino acid that has altered availability in human ulcerative colitis (UC), a form of inflammatory bowel disease, and is beneficial in murine colitis induced by dextran sulfate sodium (DSS), a model with similarity to UC.
Elena A Govorkova et al.
Antimicrobial agents and chemotherapy, 59(3), 1495-1504 (2014-12-24)
Compounds that target the cellular factors essential for influenza virus replication represent an innovative approach to antiviral therapy. Sp2CBMTD is a genetically engineered multivalent protein that masks sialic acid-containing cellular receptors on the respiratory epithelium, which are recognized by influenza
Rubia I Mancuso et al.
Immunity, inflammation and disease, 6(1), 128-142 (2017-11-10)
Streptococcus pneumoniae colonizes the nasopharynx of healthy individuals establishing a commensal relationship with the host. In some conditions, bacteria invade the lower respiratory tract and innate immune responses are crucial to avoid diseases such as pneumonia, sepsis, or meningitis. Here
Wei Lei et al.
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Sutherlandia frutescens is a medicinal plant that has been traditionally used in southern Africa for cancers, infections, and inflammatory conditions. We recently published experiments demonstrating that an aqueous extract of S. frutescens possessed potent immune-stimulatory activity. This work was carried
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It seems that the sample wells are experiencing low bead counts while the standard and control wells are unaffected. This issue is limited to 1 or 2 cytokines, while the rest show normal counts. It doesn't appear to be related to the instrument, bead loss, or inadequate bead mixing. What could be the possible reason for this discrepancy?
1 answer-
Helpful?
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Does the addition of 25 µL of Assay Buffer to the sample well count as a dilution when using a Milliplex kit that does not require a sample dilution prior to loading the plate?
1 answer-
If the sample is not diluted prior to loading the plate, and the same volume of standard and sample are loaded, then there is no need to apply a sample dilution factor.
Helpful?
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