MABS2054

Anti-ABCG8 Antibody, clone 1B10A5

clone 1B10A5, from mouse

• Synonym(s): ATP-binding cassette sub-family G member 8, Sterolin-2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1B10A5, monoclonal
• species reactivity: human, mouse, rat
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001272934
• UniProt accession no.: Q9DBM0
• target post-translational modification: unmodified
• Gene Information: mouse ... Abcg8(67470)

Description

General description

ATP-binding cassette sub-family G member 8 (UniProt: Q9DBM0, also known as Sterolin-2, ABCG8) is encoded by the Abcg8 gene (Gene ID: 67470) in murine species. ABCG8 is a multi-pass membrane protein that forms an obligate heterodimer with ABCG5 to mediate Mg2+ and ATP-dependent sterol transport across the cell membrane. It is required for normal sterol homeostasis and plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. ABCG8 and ABCG5 are primarily expressed in enterocytes and hepatocytes and their levels are up-regulated upon cholesterol feeding. They are shown to heterodimerize in the endoplasmic reticulum prior to being transported to apical membranes. Their ATPase catalytic domain is located N-terminal to the transmembrane domain. ABCG8 has an ABC transporter domain (aa 48-314) and a ABC transporter type-2 domain (aa 411-665). Two isoforms of ABCG8 have been described that are produced by alternative splicing. Inactivating mutations in either G5 or G8 are reported to cause sitosterolemia, a rare autosomal-recessive disorder characterized by hypercholesterolemia, phytosterolemia, and premature coronary artery disease. (Ref.: Graf, GA., et al. (2003). J. Biol. Chem. 278(48); 48275-48282.

Specificity

Clone 1B10A5 detects ATP-binding cassette sub-family G member 8 (ABCG8). It targets an epitope with in the N-terminal half.

Immunogen

Recombinant fragment corresponding to the first 350 amino acids from mouse ATP-binding cassette sub-family G member 8 (ABCG8).

Application

Anti-ABCG8, clone 1B10A5, Cat. No. MABS2054, is a mouse monoclonal antibody that detects ATP-binding cassette sub-family G member 8 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected ABCG8 in Immunocytochemistry applications (Wang, J., et. al. (2008). Biochemistry. 47(18):5194-204).

Immunoprecipitation Analysis: A representative lot detected ABCG8 in Immunoprecipitation applications (Graf, G.A., et. al. (2003). J Biol Chem. 278(48):48275-82).

Western Blotting Analysis: A representative lot detected ABCG8 in Western Blotting applications (Wang, J., et. al. (2008). Biochemistry. 47(18):5194-204; Graf, G.A., et. al. (2003). J Biol Chem. 278(48):48275-82).

Research Category
Signaling

Quality

Evaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected ABCG8 in mouse colon tissue sections.

Target description

75.99 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable