MABF2115

Anti-NKR-P1B (CD161b) Antibody, clone 2D12

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• antibody form: purified antibody
• clone: 2D12; monoclonal
• purified by: using protein G
• species reactivity: mouse, mouse, mouse
• concentration: (Please refer to lot specific datasheet.)
• technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aλ
• suitability: suitable for immunoassay
• UniProt accession no.: Q99JB4

Description

General description

Mouse BaF/3 cells (murine IL-3-dependent pro-B cell line) expressing mouse NKR-P1B protein.

Specificity

Clone 2D12 is a mouse monoclonal antibody that detects Natural killer cell surface protein NKR-P1B allele B6 (NKR-P1B) in murine species. It does not react with Nkrp1a, Nkrp1c, and Nkrp1f.

Application

Affects Function Analysis: A representative lot reversed the reduction in killing induced by the expression of Clrb in C1498, BW5147, and B6 LAK cells. (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).

Immunoprecipitation Analysis: A representative lot immunoprecipitated NKR-P1B (CD161b) in Immunoprecipitation applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).

Flow Cytometry Analysis: A representative lot detected NKR-P1B (CD161b) in Flow Cytometry applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7; Aguilar, O.A., et. al. (2017). Cell. 169(1):58-71).

Quality

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NKR-P1B (CD161b) in mouse spleen tissue lysate.

Physical properties

~52 kDa observed; 25.03 kDa calculated. . Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.