APG5 autophagy 5-like, APG5 autophagy 5-like (S. cerevisiae), APG5 (autophagy 5, S. cerevisiae)-like, ATG5 autophagy related 5 homolog (S. cerevisiae), ATG5 autophagy related 5-like, Apoptosis-specific protein
ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity
Specificity
Other species have not been tested.
The antibody reacts with the 50 kDa ATG5 protein.
Immunogen
Linear Peptide, aa: 8-318
Application
Immunoprecipitation: HeLa cell lysate
Research Category Apoptosis & Cancer
Research Sub Category Apoptosis - Additional
This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.
Quality
Western Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.
Target description
50 kDa
Linkage
Replaces: MABC137
Physical form
Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control Western Blot and Immunoprecipitation: HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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