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AB10029

Sigma-Aldrich

Anti-Ubiquityl Histone H2A.X (Lys119) Antibody

serum, from rabbit

Synonym(s):

H2AXK119Ub, Histone H2A.X Ubiquityl K119), H2A histone family, member X, H2AX histone

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_002096.1

UniProt accession no.

P16104

shipped in

wet ice

target post-translational modification

ubiquitination (Lys119)

Gene Information

bovine ... H2Ax(531733)
dog ... H2Ax(489372)
human ... H2AX(3014)
mouse ... H2Ax(15270)
rat ... H2Ax(500987)

General description

H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins a damaged site. H2AX is phosphorylated soon after the appearance of double-stranded breaks. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.


Specificity

This antibody recognizes Histone H2A.X when ubiquitylated at Lys119.

Immunogen

Epitope: Lys119
Linear peptide containing ubiquitylated Lys119 of human Histone H2A.X.

Application

Anti-Ubiquityl Histone H2A.X (Lys119) Antibody is a rabbit polyclonal antibody for detection of Ubiquityl Histone H2A.X (Lys119) also known as H2AXK119Ub, Histone H2A.X Ubiquityl K119) & has been validated in WB, IP, ICC.
Immunocytochemsitry Analysis: 1:500 dilution from a representative lot detected Histone H2A.X in RAW264.7 and NIH/3T3 cells.

Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.

Target description

~ 23 kDa

Physical form

Unpurified
Unpurified rabbit polyclonal containing serum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Analysis Note

Control
HeLa acid extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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