Purified mouse monoclonal antibody. Recognizes the mannose 6-phosphate receptor protein.
Recognizes the ~300 kDa mannose 6-phosphate receptor protein.
This Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) is validated for use in Immunoblotting, IF, IHC, IP for the detection of Mannose 6-Phosphate Receptor.
Immunoblotting (1 µg/ml, non-reducing, see comments)
Immunofluorescence (see comments)
Immunohistochemistry (2-5 µg/ml)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Dintzis, S., et al. 1994. J. Biol. Chem.269, 12159. Dintzis, S., and Pfeffer, S. 1990. EMBO J.9, 77.
Does not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Human molecular genetics, 24(17), 4984-4996 (2015-06-19)
Adaptor proteins (AP 1-5) are heterotetrameric complexes that facilitate specialized cargo sorting in vesicular-mediated trafficking. Mutations in AP5Z1, encoding a subunit of the AP-5 complex, have been reported to cause hereditary spastic paraplegia (HSP), although their impact at the cellular
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