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G002490

HPLC Analysis of Bilirubin and Biliverdin on Discovery® C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery C18, 10 cm × 2.1 mm I.D., 5 μm particles (569220-U)
mobile phase: [A] 10 mM ammonium acetate (pH unadjusted) [B] 90:10, methanol:(100 mM ammonium acetate)
flow rate: 0.2 mL/min
column temp.: ambient
detector: UV, 405 nm
injection: 1 μL
sample: as indicated (in 50% MeOH, 1% NH4OH)

suitability

application for HPLC

application(s)

clinical

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
    Bilirubin

analytical column

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