ZRB2712

Anti-CD48 Antibody, clone 2I13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): BCM1 surface antigen, BLAST-1, CD48 antigen, HM48-1, MRC OX-45 surface antigen, SLAM family member 2, SLAMF2, Signaling lymphocytic activation molecule 2, sgp-60
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 2I13 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 27.38 kDa
• purified by: using Protein A
• species reactivity: mouse
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_031675
• UniProt accession no.: P18181
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: mouse ... Cd48(12506)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2I13 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects CD48. It targets an epitope within 24 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of mouse CD48.

Application

Quality Control Testing

Evaluated by Western Blotting in EL4 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD48 in EL4 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD48 in lysates from RAW264.7 cells and Mouse thymus tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD48 in Mouse spleen tissue sections.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD48 in one million C57BL6 Mouse Splenocytes .

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD48 in RAW264.7 cells.

Affinity Binding Assay Analysis: A representative lot of this antibody bound CD48 peptide with a KD of 2.6 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

CD48 antigen (UniProt: P18181; also known as BCM1 surface antigen, BLAST-1, HM48-1, MRC OX-45 surface antigen, SLAM family member 2, SLAMF2, Signaling lymphocytic activation molecule 2, sgp-60, CD48) is encoded by the Cd48 (also known as Bcm-1) gene (Gene ID: 12506) in murine species. CD48 is a GPI-anchored cell surface glycoprotein that is synthesized with a signal peptide (aa 1-22) and a propeptide (aa 218-240 that are subsequently cleaved off to produce the mature protein. It contains two Ig-like C2 type domains (aa 29-125 and 129-209). CD48 exists in both a membrane-bound and soluble form. It interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD2 to regulate immune cell function and activation. It participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex. In turn that promotes LCK phosphorylation and subsequent activation. It induces the phosphorylation of the cytoplasmic immunoreceptor tyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells. CD48 expression is observed in mature lymphocytes and monocytes, but not on non-hematopoietic cells. Its expression is increased under inflammatory conditions. It is reported to be constitutively expressed at high levels on almost all multiple myeloma cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kato, K., et al. (1992). J. Exp. Med. 176(5); 1241-1249; Moran, M., and Miceli, MC. (1998). Immunity. 9(6); 787-796).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable