R4903

Anti-hnRNP-L antibody, Mouse monoclonal

clone 4D11, purified from hybridoma cell culture

• Synonym(s): Anti-Heterogeneous Nuclear Ribonucleoprotein-L
• MDL number: MFCD03845440
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 4D11, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~68 kDa
• species reactivity: bovine, newt, chicken, human, monkey, mouse
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of MDBK cells
• isotype: IgG1
• UniProt accession no.: P14866
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HNRNPL(3191) ; mouse ... Hnrnpl(15388)

Description

General description

Monoclonal Anti-hnRNP-L (mouse IgG1 isotype) is derived from the 4D11 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALA/c mice immunized with hnRNP proteins and purified by affinity chromatography on ssDNA-agarose. RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. Group L and M are one of the most abundant groups of proteins.

Immunogen

hnRNP proteins.

Application

Anti-hnRNP-L antibody has been used in

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation
• immunocytochemistry
• western blotting

Biochem/physiol Actions

Heterogeneous nuclear ribonucleoproteins-L (hnRNP-L) is involved in splicing regulations, internal translation initiation of hepatitis C virus (HCV), stability of the human vascular endothelial growth factor (VEGF) mRNA under hypoxic growth conditions, and intron independent mRNA export.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves