E3763

Resorufin ethyl ether

≥98% (TLC), powder

• Synonym(s): 7-Ethoxy-3H-phenoxazin-3-one, Ethoxyresorufin, O⁷-Ethylresorufin
• Empirical Formula (Hill Notation): C₁₄H₁₁NO₃
• CAS Number: 5725-91-7
• Molecular Weight: 241.24
• Beilstein: 225973
• MDL number: MFCD00037661
• UNSPSC Code: 12161501
• PubChem Substance ID: 24894527
• NACRES: NA.47

Properties

• Quality Level: 200
• Assay: ≥98% (TLC)
• form: powder
• storage condition: (Tightly closed. Dry)
• technique(s): activity assay: suitable
• color: orange to red
• mp: 223-225 °C (lit.)
• solubility: chloroform: 9.80-10.20 mg/mL, clear, orange
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CCOc1ccc2N=C3C=CC(=O)C=C3Oc2c1.Fc4c(F)c(F)c(OC(=O)CNC(=O)OCC5c6ccccc6-c7ccccc57)c(F)c4F
• InChI: 1S/C23H14F5NO4.C14H11NO3/c24-17-18(25)20(27)22(21(28)19(17)26)33-16(30)9-29-23(31)32-10-15-13-7-3-1-5-11(13)12-6-2-4-8-14(12)15;1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h1-8,15H,9-10H2,(H,29,31);3-8H,2H2,1H3
• InChI key: ZOSYTBPPLWBBKM-UHFFFAOYSA-N

Description

General description

Resorufin ethers are used as markers or substrates for different cytochrome P (CYP) isoforms. Resorufin ethyl ether is the preferred resorufin ether to be metabolized by microsomes of 3-methylcholanthrene treated animals.[1]

Resorufin is the reduced product of resazurin, also termed as Alamar Blu.[2] It exhibits fluorescence at longer wavelengths and is flexible for modification in its hydroxyl group.[3]

Research area: Cell Signaling[3]

Application

Resorufin ethyl ether is suitable for the study of dealkylating activity of cytochrome P450 isozymes 1A1 and 1A2, where arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and A′-acetylcysteine conjugates are used as inhibitors.[4] It is suitable to study the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 activity (CYP1A1).[5]

Substrates

Fluorimetric substrate for cytochrome P450 linked enzyme, CXPIA1/2

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves