biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS1
Reactivity
Anti-WC3, bovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS1 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against the WC3 antigen present on bovine B cells. The antigen has a molecular weight of 145kDa. The antibody will also react with the equivalent antigen in sheep and goats.
Immunogen
Bovine macrophages
Culture Medium
RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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Certificates of Analysis (COA)
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