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C7594

Sigma-Aldrich

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Synonym(s):

blocking buffer

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About This Item

EC Number:
232-555-1
UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

form

powder blend

technique(s)

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

storage temp.

room temp

Related Categories

General description

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Application

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

Preparation Note

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

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Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
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Sambrook, J., et al.
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Protocols

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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