C5491
Cellytic™ Express, 1 mL Tablets
1 tablet sufficient for 1 mL lysis (cell suspension)
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About This Item
UNSPSC Code:
41105500
NACRES:
NA.56
Recommended Products
form
tablet
Quality Level
usage
tablet sufficient for 1 mL lysis (cell suspension)
storage temp.
−20°C
Application
All the advantages of CelLytic™ Express now in a tablet format. For direct lysis of bacterial cultures and for use in the His-Select® iLAP™ column. CelLytic Express tablets are a complete formulation including lysozyme and endonuclease for complete cell lysis. The resulting lysate is clear of cellular debris, and is immediately ready for standard affinity purification. This new formulation adds minimal volume to the final lysate, providing a method with defined volume and time parameters, making it easier to validate and making it more convenient for production scale protocols.
Features and Benefits
- Efficient and non-denaturing in-culture protein extraction
- Save time by eliminating cell harvest and clarification steps
- Preserve biological activity with fewer manipulations
- Compatible with affinity resins and magnetic beads
- Ideal for Production Scale Extraction protocols
Unit Definition
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
iLAP is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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Han-Shin Kim et al.
Biotechnology and bioengineering, 118(1), 82-93 (2020-09-04)
Bacterial biofilm formation causes serious problems in various fields of medical, clinical, and industrial settings. Antibiotics and biocide treatments are typical methods used to remove bacterial biofilms, but biofilms are difficult to remove effectively from surfaces due to their increased
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