A3733
Bovine Serum Albumin
lyophilized powder, ≥98% (agarose gel electrophoresis)
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Recommended Products
biological source
bovine
Assay
≥98% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
packaging
poly bottle of
origin
New Zealand origin
technique(s)
flow cytometry: suitable
pH
7
~7 (1% in 0.15 M NaCl)
solubility
water: slightly soluble
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
Bovine serum albumin (BSA) that belongs to the serum albumin family, is an α-helical, globular and non-glycosylated protein. It comprises three domains with two subdomains under each domain.
Application
Bovine Serum Albumin has been used:
- as a component of cryomedia for freezing testicular tissue
- to resuspend myometrial cells in flow cytometric analysis for cell viability quantification
- to block cardiac fibroblasts (CFs) for immunofluorescence staining
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is a transporter for drugs, hormones, and fatty acids. It displays high solubility and is exploited in the cosmetic and pharmaceutical industries. BSA is useful as a cell media component and favors embryonic stem cells (hESC)differentiation.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Derived from New Zealand source serum
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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