78361

Fluorescent red 630 reactive

for fluorescence, ≥80% (coupling to amines)

• Synonym(s): Reactive fluorescent red 630
• Empirical Formula (Hill Notation): C₄₀H₄₉N₃O₈S
• CAS Number: 350496-73-0
• Molecular Weight: 731.90
• MDL number: MFCD03456833
• UNSPSC Code: 12171500
• PubChem Substance ID: 329768140

Properties

• grade: for fluorescence
• Assay: ≥80% (coupling to amines)
• solubility: DMF: soluble
• fluorescence: λ_ex 620 nm; λ_em 650 nm in 0.1 M phosphate pH 7.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CCN(CC)c1ccc2c(\C=C\C=C3\N(CCCCCC(=O)ON4C(=O)CCC4=O)c5ccc(cc5C3(C)C)S([O-])(=O)=O)cc([o+]c2c1)C(C)(C)C
• InChI: 1S/C40H49N3O8S/c1-8-41(9-2)28-17-19-30-27(24-35(39(3,4)5)50-33(30)25-28)14-13-15-34-40(6,7)31-26-29(52(47,48)49)18-20-32(31)42(34)23-12-10-11-16-38(46)51-43-36(44)21-22-37(43)45/h13-15,17-20,24-26H,8-12,16,21-23H2,1-7H3/b14-13+,34-15+
• InChI key: PHHUECZJTSYKRH-DBYPPFPASA-N

Description

Application

Amino-reactive biolabel excited by red lasers (He/Ne; 633 nm) and diode lasers (635-650 nm). The chromophore is a bridged hemicyanine. Quantum yield is fairly low in aqueous media, but increases up to one order of magnitude if coupled to a target protein; Molar absorption 120,000 M^-1 cm^-1, λ_abs: (conjugate) 645 nm; λ_em: (conjugate) 660 nm

Features and Benefits

This novel hemocyanin dye is optimally excited by He-Ne laser with a wavelength of 633 nm, but may also be excited by argon or argon-krypton laser with a wavelength of 647 nm or diode lasers.
Additional advantages are:
High molar absorptions (120,000 M^-1cm^-1)
Low quantum yields in nonaqueous media, greatly increased by binding to target molecules.
Very strong fluorescence in solid state, which allows preparation of DNA for arrays on glass.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves