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69965

Millipore

Mossel Broth

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Buffered Glucose-Brilliant Green Bile Broth, EE Broth, Enterobacteriaceae Enrichment Broth acc. to Mossel

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

brilliant green, 0.015 g/L
disodium hydrogen phosphate, 6.45 g/L
glucose, 5 g/L
ox-bile, 20 g/L
peptone, 10 g/L
potassium dihydrogen phosphate, 2 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (20 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective for Citrobacter spp.
selective for Enterococcus spp.
selective for Escherichia coli
selective for Klebsiella spp.
selective for Proteus spp.
selective for Salmonella spp.
selective for Shigella spp.
selective for Yersinia spp.
selective for coliforms
selective for enterobacteriaceae

Related Categories

Application

For the selective enrichment of all enterobacteriaceae from food and other material.

Preparation Note

Dissolve 43.5 g in 1 litre distilled water and heat at 100°C in water bath or flowing steam for 30 minutes. Cool quickly. This medium is heat sensitive. DO NOT AUTOCLAVE.

Other Notes

Isolation of Serratia marcescens from clinical samples

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCK6125
BCCH1262
BCCD3889
BCBW9239
BCBT6416

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G.M. Grasso et al.
Epidem. Inf., 101, 315-315 (1988)
D.A.A. Mossel et al.
The Journal of Applied Bacteriology, 26, 444-444 (1963)

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Cronobacter spp. are frequently found on vegetables, meat, fermented bread, dairy and in baby food. Consumption of contaminated powdered infant formula milk can result in sepsis, infant meningitis and necrotizing enterocolitis.

Differentiation and ID Media for Salmonella

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Protocols

Enterobacteriaceae in Food

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

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