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08714

Millipore

Methyl Red solution

suitable for microbiology

Synonym(s):

Methyl red indicator solution

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About This Item

Empirical Formula (Hill Notation):
C15H15N3O2
CAS Number:
493-52-7
Molecular Weight:
269.30
Beilstein:
1843037
MDL number:
MFCD00002425
UNSPSC Code:
41171621
PubChem Substance ID:
329748627
NACRES:
NA.85

Agency

according to GB 4789.30-2016
according to ISO 22964:2017

Quality Level

200

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

dist. water, 200 mL
ethanol 95%, 300 mL
methyl red, 0.1 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

Enterococcus spp.
Escherichia coli
Klebsiella spp.
Proteus spp.
Pseudomonas spp.
Staphylococcus spp.
bacteria

SMILES string

CN(C)c1ccc(cc1)\N=N\c2ccccc2C(O)=O

InChI

1S/C15H15N3O2/c1-18(2)12-9-7-11(8-10-12)16-17-14-6-4-3-5-13(14)15(19)20/h3-10H,1-2H3,(H,19,20)/b17-16+

InChI key

CEQFOVLGLXCDCX-WUKNDPDISA-N

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General description

Methyl red solution is an azo dye which turns to red when pH is below 4.4 (yellow pH < 6.2, orange pH 4.4-6.2). Some bacteria utilize glucose to form large amounts of acid with the result that the pH value of the medium falls distinct. Other species produce no or less free acid. This difference can be visualized by using methyl red. This test is used to differentiate enteric bacteria.

Application

Methyl red solution is a pH indicator dye recommended for detection of bacteria producing acid from glucose fermentation such as Enterobacteriaceae in various samples.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

69.8 °F

Flash Point(C)

21 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL8848
BCCL0746
BCCK1481
BCCJ3868
BCCG7250

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Angel Martinez et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(52), 20891-20896 (2011-12-14)
Noncontact optical trapping and manipulation of micrometer- and nanometer-sized particles are typically achieved by use of forces and torques exerted by tightly focused high-intensity laser beams. Although they were instrumental for many scientific breakthroughs, these approaches find few technological applications
C E Tye et al.
Journal of dental research, 89(12), 1421-1426 (2010-10-12)
During enamel maturation, hydroxyapatite crystallites expand in volume, releasing protons that acidify the developing enamel. This acidity is neutralized by the buffering activity of carbonic anhydrases and ion transporters. Less hydroxyapatite forms in matrix metalloproteinase-20 null (Mmp20(-/-)) mouse incisors, because
Taiga Fujii et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(42), 13304-13313 (2012-09-22)
We prepared reversed dye clusters by hybridizing two RNA oligomers, each of which tethered dyes (Methyl Red, 4'-methylthioazobenzene, and thiazole orange) on D-threoninols (threoninol nucleotides) at the center of their strands. NMR spectroscopic analyses revealed that two dyes from each
Improvement of RNAi activity and strand selectivity of RISC formation by modified siRNA involving intercalators near 5' termini.
Hiroshi Ito et al.
Chembiochem : a European journal of chemical biology, 13(2), 311-315 (2012-01-04)
Chan-Ju Wang et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 66(Pt 1), 2-7 (2010-01-09)
Azoreductase 1 from Pseudomonas aeruginosa strain PAO1 (paAzoR1) catalyses the activation of the prodrug balsalazide and reduces the azo dye methyl red using reduced nicotinamide adenine dinucleotide cofactor as an electron donor. To investigate the mechanism of the enzyme, a
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