MABS1713

Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2

clone 7D2.2, from mouse

• Synonym(s): Synaptotagmin-like protein 4, Exophilin-2, Granuphilin
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7D2.2, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001123368
• UniProt accession no.: Q96C24
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... SYTL4(94121)

Description

General description

Synaptotagmin-like protein 4 (UniProt Q96C24; also known as Exophilin-2, Granuphilin) is encoded by the SYTL4 (also known as SLP4) gene (Gene ID 94121) in human. SYLT4 belongs to the synaptotagmin-like protein family, which contains an N-terminal RabBD (Rab-binding) domain and two C-terminal C2 domains. The characteristic feature of this family is the N-terminal domain, which is not found in other C-type tandem C2 proteins. SYLT4 is detected close to the plasma membrane and on secretory granules. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner. It can bind to both the GTP- and GDP-bound forms of Rab27A and inhibits a specific GTP/GDP exchange cycle required for dense-core vesicle exocytosis. It is the first regulatory in the exocytosis pathway that functions by directly connecting Rab and SNARE. It modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. It is not only essential for the docking of insulin granules, but also simultaneously imposes a fusion constraint on them through an interaction with syntaxin-1alpha fusion machinery.

Specificity

Clone 7D2.2 epitope resides within an internal region mostly missing in human spliced isoform 2. Reactivity toward isoform 2 has not been confirmed.

Immunogen

GST-tagged recombinant human synaptotagmin-like protein 4 (SYTL4) internal fragment.

Application

Detect SYTL4 using this mouse monoclonal Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2, Cat. No. MABS1713, validated for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected synaptotagmin-like protein 4 (SYTL4) in 10 µg of human adrenal gland tissue lysate.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected synaptotagmin-like protein 4 (SYTL4) in 10 µg of HeLa cell lysate.

Target description

~80 kDa observed. 70.02 kDa (human isoform 1) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1