ABC961
Anti-OFD1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Oral-facial-digital syndrome 1 protein homolog, OFD1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
rat ... Ofd1(302661)
General description
The cilium is a cell surface projection found in many vertebrate cells and it is required to transduce signals important for development and tissue homeostasis. OFD1 is important in cilium development, and thus it is important in cellular development and signaling. OFD1 is a component of the centrioles and appears to control the length of the centrioles in mother-daughter cells. OFD1 also plays an important role in the WNT signaling pathway and in the formation of the left-right axis in embryos. OFD1 is expressed in the cytoplasm during development in most tissues but can be found in the nucleus as well, and it is known to interact with proteins important in chromatin remodeling as well. At least one X-linked genetic mutation is associated with defects in the OFD1 gene. It is a form of oroffaciodigital syndrome characterized by abnormalities in the oral cavity, face, digits and other disfigurements that is lethal in males.
Immunogen
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of mouse OFD1.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-OFD1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of OFD1.
Western Blotting Analysis: A representative lot detected OFD1 in untreated and autophagy-induced/serum-starved mouse MEFs (Tang, Z., et al. (2013). Nature. 502:254-257).
Western Blotting Analysis: A representative lot detected OFD1 in wild-type female mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Western Blotting Analysis: A representative lot detected OFD1 in male E10.5 knock-out mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Immunocytochemistry Analysis: A representative lot detected OFD1 in PCM1 knockdown U2OS cells & untreated and serum-starved/autophagy-induced U2OS cells where OFD1 partially colocalizes with LC3 (Tang, Z., et al. (2013). Nature. 502:254-257).
Western Blotting Analysis: A representative lot detected OFD1 in wild-type female mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Western Blotting Analysis: A representative lot detected OFD1 in male E10.5 knock-out mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Immunocytochemistry Analysis: A representative lot detected OFD1 in PCM1 knockdown U2OS cells & untreated and serum-starved/autophagy-induced U2OS cells where OFD1 partially colocalizes with LC3 (Tang, Z., et al. (2013). Nature. 502:254-257).
Quality
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OFD1 in 10 µg of U2OS cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OFD1 in 10 µg of U2OS cell lysate.
Target description
~120 kDa observed. Uncharacterized bands may be observed in some lysates at 45 kDa, 33 kDa & 28 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Noémie Gaudin et al.
eLife, 11 (2022-03-24)
Centrioles are formed by microtubule triplets in a ninefold symmetric arrangement. In flagellated protists and animal multiciliated cells, accessory structures tethered to specific triplets render the centrioles rotationally asymmetric, a property that is key to cytoskeletal and cellular organization in
Emanuela Senatore et al.
The EMBO journal, 40(10), e106503-e106503 (2021-05-03)
The primary cilium is a microtubule-based sensory organelle that dynamically links signalling pathways to cell differentiation, growth, and development. Genetic defects of primary cilia are responsible for genetic disorders known as ciliopathies. Orofacial digital type I syndrome (OFDI) is an
Hideki Izumi et al.
Scientific reports, 9(1), 2236-2236 (2019-02-21)
CD133 is a transmembranous protein that mainly localises to the plasma membrane in haematopoietic and neural stem cells as well as cancer stem cells. Although CD133 also localises to the cytoplasm, the mechanism of action and function of cytoplasmic CD133
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