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Supelco

Purospher® STAR Phenyl (3 µm) HPLC Columns

L × I.D. 100 mm x 2.1 mm Hibar® HR UHPLC Column

Synonym(s):

Purospher® STAR Phenyl, 3µm Hibar® HR 100-2.1

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Purospher® STAR Phenyl (3 µm), L × I.D. 100 mm x 2.1 mm Hibar® HR UHPLC column

material

stainless steel column

Quality Level

Agency

suitable for USP L11

product line

Purospher®

feature

endcapped: no

packaging

1 ea of

extent of labeling

12.5% Carbon loading

parameter

1000 bar pressure
65 °C max. temp.

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

matrix

fully porous particle

matrix active group

Phenyl bonding phase

particle size

3 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

General description

Purospher® STAR Phenyl, 3 µm UHPLC columns are the best alternative to RP-8 or RP-18 columns for the separation of aromatic compounds, and compounds containing aromatic groups. Purospher® STAR Phenyl can retain analytes via several different mechanisms, including ο-ο interactions between the overlap of the delocalized electrons on the analyte and the stationary phase phenyl group, and via partitioning between the mobile phase and the hydrophobic aryl-alkyl phase. Our Purospher® STAR Phenyl columns are based on highpurity silica particles, which provide symmetrical peaks for basic compounds, as well as high stability and excellent reproducibility. Furthermore, hydrophobic compounds elute much faster with Purospher® STAR Phenyl columns than with C-18 columns.Purospher® STAR Phenyl, 3 µm Hibar® HR 100-2.1 is a ready to use UHPLC column suitable for connection to any UHPLC system providing a pressure stability of 1000 bar.The column dimension of 100 mm length x 2.1 mm I.D. is the optimal choice for fast UHPLC separation of complex samples allowing significant solvent savings and improved sensitivity. A 2.1 mm I.D. column enables a more sustainable separations in comparison to 4, 4.6 or 3 mm I.D. column due to the use of significant less solvent for the chromatographic separation.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of antibiotics, aromatic amines, flavonoids, ketones, nucleosides, PAHs, peptides, preservatives, steroids, metabolized steroids, and sweeteners.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Enhanced selectivity for aromatic compounds
  • Suitable for use with highly aqueous mobile phases
  • Good suitability for LC-MS applications

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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