1.50657

SeQuant^® ZIC^®-cHILIC 100Å (3 µm) HPLC Columns

L x I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

• Synonym(s): Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: SeQuant^® ZIC^®-cHILIC 3µm,100Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
• material: PEEK-lined stainless steel column
• Quality Level: 100
• product line: SeQuant^®
• feature: endcapped: no
• parameter: 550 bar pressure; 70 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 10 cm × 2.1 mm
• matrix: fully porous particle
• matrix active group: ZIC-cHILIC bonding phase
• particle size: 3 μm
• pore size: 100 Å pore size
• operating pH: 1-7.5
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Description

General description

SeQuant^® ZIC^®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.The column dimension of 100 mm length x 2.1 mm ID is the best choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.SeQuant^® ZIC^®-cHILIC HPLC columns provide complementary selectivity to optimize separations of polar hydrophilic compounds. The columns carry a novel densely bonded, 1:1 charge-balanced phosphorylcholine functional group. As with all true zwitterionic stationary phases, straightforward and reproducible separations are achieved by hydrophilic partitioning combined with weak ionic interactions.The more accessible positive charges of SeQuant^® ZIC^®-cHILIC columns enable selectivity tuning in chromatography through altered interaction with anionic and cationic molecular moieties. This can be favorable in several situations, for example in the separation of labile metal complexes or highly polar and multiply charged aminoglycosides. Their extraordinary stability and minimized bleed also make SeQuant^® ZIC^®-cHILIC columns the preferred choice for many LC-MS applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Pressure: ≤ 40 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany