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Quality Level
form
liquid
IVD
for in vitro diagnostic use
transition temp
flash point 19 °C
density
0.892 g/cm3 at 20 °C
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
Related Categories
General description
Eosin Y solution 1%, alcoholic - for microscopy is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin in histological and clinico-cytological specimen materials, for example histological sections of e. g. the kidney, muscle tissue, heart, or lung. The hematoxylin and eosin (H&E) staining method is the method most frequently used for the staining of histology material. The staining mechanism behind the H&E staining is a physico-chemical process. In the first step, the positively charged nuclear dye (hematoxylin) binds to the negatively charged phosphate groups of the nucleic acid of the cell nucleus. The nuclei will be dyed dark blue to dark violet. The second step is the counterstaining with negatively charged anionic Eosin Y, a xanthene dye. Eosin binds to the positively charged plasma proteins. Cytoplasma and intercellular substances are stained pink to red, while erythrocytes will appear with yellow to orange color.
One 500ml bottle of the stain provides 1250 stainings. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
One 500ml bottle of the stain provides 1250 stainings. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
Analysis Note
Suitability for microscopy (Tissue section): passes test
Nuclei: dark blue to dark violet
Cytoplasm: pink to red
intercellular substances: pink to red
Erythrocytes: yellow to orange
Nuclei: dark blue to dark violet
Cytoplasm: pink to red
intercellular substances: pink to red
Erythrocytes: yellow to orange
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Flash Point(F)
66.2 °F
Flash Point(C)
19 °C
Certificates of Analysis (COA)
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Mare Mechelinck et al.
PloS one, 14(12), e0226430-e0226430 (2019-12-14)
A simple but reliable and safe anaesthetic procedure is required for surgical interventions in small rodents. Combined ketamine and xylazine injections are often used in rats for less invasive surgery, possibly with spontaneous breathing and without airway management. However, there
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