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103785

Millipore

Egg yolk tellurite emulsion

pasteurized, 20%, suitable for microbiology

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About This Item

UNSPSC Code:
41106212

biological source

chicken egg yolk

Quality Level

200

Agency

APHA
ISO 6888

reg. compliance

FDA (BAM)

form

liquid

concentration

20%

density

1.02 g/cm3 at 20 °C

application(s)

food and beverages
microbiology

shipped in

ambient

storage temp.

2-8°C

General description

Egg-yolk tellurite emulsion is a stabilized emulsion of egg yolk for microbiological use, which is added directly to nutrient media for the identification of strains of coagulase-positive Staphylococci by their lecithinase activity. Potassium tellurite has a selective activity and allows the growth of Staphylococcus as black colonies. Egg-yolk permits the detection of microbial lecithinase activity and tellurite reduction.

Application

Egg-yolk tellurite emulsion is an additive consisting of an emulsion of egg-yolk used as a supplement to prepare Baird Parker agar base medium for the detection of Staphylococcus.

Linkage

Replaces: 1.03785.0007

Analysis Note

Appearance: pale yellow emulsion
Sterility test: sterile
Typical composition: Sterile egg-yolk 200 ml; NaCl 4.25 g; potassium tellurite 2.1 g; distilled water to give a final volume of 1000 ml.
Egg-yolk Tellurite Emulsion tested in BAIRD-PARKER-Agar Art. 1.05406.
Inoculum on reference medium
- Staphylococcus aureus ATCC 25923 (WDCM 00034):
10 - 100
- Staphylococcus aureus ATCC 6538 (WDCM 00032): 10 - 100
Inoculum
- Escherichia coli ATCC 25922 (WDCM 00013):
>10⁴
- Escherichia coli ATCC 8739 (WDCM 00012): >10⁴
- Staphylococcus saprophyticus ATCC 15305 (WDCM 00159): >10⁴
- Staphylococcus epidermidis ATCC 12228 (WDCM 00036): >10⁴
Recovery on test medium
- Staphylococcus aureus ATCC 25923 (WDCM 00034):
≥ 70%
- Staphylococcus aureus ATCC 6538 (WDCM 00032): ≥ 70%
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Staphylococcus saprophyticus ATCC 15305 (WDCM 00159): poor to fair
- Staphylococcus epidermidis ATCC 12228 (WDCM 00036): poor to fair
Colony colour
- Staphylococcus aureus ATCC 25923 (WDCM 00034):
black
- Staphylococcus aureus ATCC 6538 (WDCM 00032): black
- Staphylococcus saprophyticus ATCC 15305 (WDCM 00159): black-grey
- Staphylococcus epidermidis ATCC 12228 (WDCM 00036): black-grey
Clear zones
- Staphylococcus aureus ATCC 25923 (WDCM 00034):
+
- Staphylococcus aureus ATCC 6538 (WDCM 00032): +
- Staphylococcus saprophyticus ATCC 15305 (WDCM 00159): -
- Staphylococcus epidermidis ATCC 12228 (WDCM 00036): -
Incubation: 24 - 48 hrs. at 37°C, E.coli: 48 hrs. Reference: Tryptic soy agar.

Other Notes

Strain origin: CECT® 4972

Other cell culture collections:

DSM 17076

ATCC® 700728

NCTC 12900

Legal Information

ATCC is a registered trademark of American Type Culture Collection
CECT is a registered trademark of Universitat de Valencia

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B Tenderis et al.
International journal of food microbiology, 321, 108560-108560 (2020-02-23)
Foodborne illnesses affect the health of consumers worldwide, and thus searching for potential antimicrobial agents against foodborne pathogens is given an increased focus. This research evaluated the influence of sodium lactate (SL), encapsulated (e) and unencapsulated (u) polyphosphates (PP; sodium

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