The actual yield of purified FLAG-tagged protein from a 10 cm dish using the CELLMM2 kit will depend on the expression level of the protein in the cells. The kit’s resin can bind up to 600–1000 µg per 1 mL, but typical yields from mammalian cells are often in the 100–500 µg range per dish. For precise yield, measuring the protein concentration in the lysate before and after purification is recommended.
CELLMM2
FLAG® M Purification Kit
For Mammalian expression systems.
Synonym(s):
Anti-ddddk, Anti-dykddddk
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
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This Item | TP0010 | FLAGIPT1 | E6155 |
|---|---|---|---|
| technique(s) protein extraction: suitable | technique(s) - | technique(s) immunoprecipitation (IP): suitable | technique(s) - |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in dry ice |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
Application
The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Features and Benefits
- Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
- ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
- Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).
Packaging
Sufficient for 3-5 uses of 1-ml affinity purification column.
Other Notes
Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
Product No.
Description
SDS
- C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
- SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powder
- A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution
- C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.
Storage Class
10 - Combustible liquids
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