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58201

Supelco

SUPELCOSIL™ LC-Diol (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

SUPELCOSIL™ LC-DIOL 5UM 25CMX4.6MM HPLC COLUMN

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
Pricing and availability is not currently available.

Product Name

SUPELCOSIL™ LC-Diol HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L20

feature

endcapped: no

packaging

pkg of 1 ea

manufacturer/tradename

SUPELCOSIL™

extent of labeling

3.5% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.8 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

diol phase

particle size

5 μm

pore size

120 Å pore size

pH range

2-7.5

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H371

Hazard Classifications

STOT SE 2 Inhalation

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
294526-04
294526-03
294526-02
294526-01
294525-04

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Questions

1–6 of 6 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Can Supelcosil LC-Diol HPLC column, Product 58201, be use with acid?

    1 answer

    1. No. The SUPELCOSIL LC-DIOL is very susceptible to cleaving of the phase using acids, therefore we do not recommend using acids, not even 1% acetic acid.

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  5. Can Supelcosil LC-Diol HPLC column, Product 58201, be used in a reversed-phase and normal phase system?

    1 answer

    1. Yes. Before moving from one mode to another, the column should be flushed thoroughly with IPA (isopropyl alcohol).

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  6. What is the phase for Supelcosil LC-Diol HPLC column, Product 58201?

    1 answer

    1. The SUPELCOSIL LC-DIOL phase is a glycidoxypropylsilyl.

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