This antibody targets an epitope located in amino acid residues 4-10 at the N-terminus of the Opsin (rhodopsin) protein. As the retinal group is covalently linked to the opsin protein component on its lysine residues and the epitope region (AA 4-10) does not contain any lysine, it is unlikely that a retinal group is involved in the reactivity of this antibody. Therefore, the reactivity of this antibody would only involve the opsin component of the observed rhodopsin protein (UniProt#: P08100) upon labeling.
SAB4200762
Anti-Opsin antibody, Mouse monoclonal
clone RET-P1, purified from hybridoma cell culture
Synonym(s):
Anti-Opsin 2, Anti-Rhodopsin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.
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biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
RET-P1, monoclonal
form
buffered aqueous solution
mol wt
~39 kDa
species reactivity
avian, bovine, fish, mouse, rat, human, amphibian, turtle, rabbit
concentration
~1 mg/mL
technique(s)
ELISA: suitable
immunoblotting: 2-4 μg/mL using human retinoblastoma Y79 cell line extract.
immunofluorescence: suitable
immunohistochemistry: 5-10 μg/mL using rat eye frozen sections.
radioimmunoassay: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Rho(24717)
Related Categories
General description
Anti-Opsin antibody, Mouse monoclonal (rhodopsin) (mouse IgG1 isotype) is derived from the RET-P1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/C mice immunized with rat retinal membranes. Opsin is also called as rhodopsin protein, members of the G-protein coupled receptor (GPCR) superfamily. Opsin comprises >95% of the rod outer segments (ROS) intrinsic membrane and consists of a protein moiety - an opsin - and a non-protein moiety - the chromophore retinal.
Application
Anti-Opsin antibody has been used in
- immunoblotting
- immunofluorescence
- immunohistochemistry
- enzyme linked immunosorbent assay (ELISA)
- radioimmuno assay (RIA)
Biochem/physiol Actions
Opsin is an essential molecules for mediating the ability of animals to detect and use light for diverse biological functions. Mutations in the opsin (rhodopsin) gene are linked to retinitis pigmentosa (RP), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness.
Other Notes
rat retinal membranes
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Retinitis pigmentosa
Hamel C.
Orphanet Journal of Rare Diseases, 1(1), 40-40 (2006)
The opsins
Terakita A.
Genome Biology, 6(3), 213-213 (2005)
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I am confused by the wording in the description of the product on your website: "Anti-Opsin antibody, Mouse monoclonal (rhodopsin) (mouse IgG1 isotype) is derived from the RET-P1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/C mice immunized with rat retinal membranes. Opsin is also called as rhodopsin protein, members of the G-protein coupled receptor (GPCR) superfamily. Opsin comprises >95% of the rod outer segments (ROS) intrinsic membrane and consists of a protein moiety - an opsin - and a non-protein moiety - the chromophore retinal." Does this imply that this anti-opsin will recognize Rhodopsin as well, i.e. when we observe labeling for opsin using this antibody we could be observing Rhodopsin?
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