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PLA0201

Sigma-Aldrich

Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):

14-3-3 sigma, Stratifin, YWHAS, epithelial cell marker protein 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

human

technique(s)

immunohistochemistry: 1:2,000-1:10,000
immunoprecipitation (IP): 2-5 μg/mg
western blot: 1:2,000- 1:10,000

accession no.

NP_006133.1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... SFN(2810)

General description

Rabbit anti-14-3-3 Sigma or stratifin is mapped to human chromosome 1p36.11. 14-3-3σ protein is expressed as seven isoforms and has molecular weight in the range of 25 to 30 kDa. It is an acidic homodimeric protein and harbors pleckstrin homology (PH) domain. 14-3-3σ is highly expressed in epidermis.

Immunogen

The epitope recognized by PLA0201 maps to a region between residue 198 and 248 of human 14-3-3 sigma using the numbering given in entry NP_006133.1 (GeneID 2810).

Application

Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified has been used in the pull down assay for Rho associated coiled-coil containing protein kinase 1 (ROCK1) proteins and heat shock 70 kDa 4 protein (Hsp74) in HCT116 cancer cells.

Biochem/physiol Actions

Rabbit anti-14-3-3 or stratifin participates in several biological events including apoptosis and cell differentiation. It regulates the transcription matrix metallopeptidases (MMPs) in fibroblasts and may serve as therapeutic potential in wound healing. 14-3-3σ is less expressed and is hyper methylated in breast cancer cells. It plays a key role in linking signalling proteins and in the enhancing of protein kinase C functionality. A mutation in the stratifin gene is implicated in epilation phenotype in mice. It interacts with ubiquitin-specific protease 8 (USP8) and is highly expressed in human lung adenocarcinoma.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A mutation in stratifin is responsible for the repeated epilation (Er) phenotype in mice
Herron BJ, et al.
Nature Genetics, 37(11), 1210-1210 (2005)
The role of stratifin in fibroblast-keratinocyte interaction
Medina A, et al.
Molecular and Cellular Biochemistry, 305(1-2), 255-264 (2007)
Hsp74/14-3-3sigma Complex Mediates Centrosome Amplification by High Glucose, Insulin, and Palmitic Acid
Lu YC, et al.
Proteomics, 1800197-1800197 (2019)
Stratifin regulates stabilization of receptor tyrosine kinases via interaction with ubiquitin-specific protease 8 in lung adenocarcinoma
Kim Y, et al.
Oncogene, 37(40), 5387-5387 (2018)
Mutation analysis of FANCD2, BRIP1/BACH1, LMO4 and SFN in familial breast cancer
Lewis AG, et al.
Breast Cancer Research, 7(6), R1005-R1005 (2005)

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