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Key Documents

N3786

Sigma-Aldrich

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Proteomics Grade, suitable for MALDI-TOF MS

Synonym(s):

Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

form

lyophilized powder

Quality Level

quality

Proteomics Grade

specific activity

≥25 U/vial

suitability

suitable for MALDI-TOF MS

storage temp.

2-8°C

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Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Packaging

Provided with 5× concentrate reaction buffer.

Quality

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Unit Definition

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

Physical form

Lyophilized powder

Kit Components Also Available Separately

Product No.
Description
SDS

  • N3536Neuraminidase 5X Reaction Buffer 1.5 mLSDS

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Description
Pricing

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Acta neuropathologica communications, 6(1), 92-92 (2018-09-14)
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Hongyun Li et al.
PloS one, 11(2), e0148238-e0148238 (2016-02-02)
Recent studies have shown that cerebral apoD levels increase with age and in Alzheimer's disease (AD). In addition, loss of cerebral apoD in the mouse increases sensitivity to lipid peroxidation and accelerates AD pathology. Very little data are available, however
Dennis Schade et al.
Journal of medicinal chemistry, 57(3), 759-769 (2014-01-16)
With the emergence of oseltamivir-resistant influenza viruses and in view of a highly pathogenic flu pandemic, it is important to develop new anti-influenza agents. Here, the development of neuraminidase (NA) inhibitors that were designed to overcome resistance mechanisms along with
Michael Worobey et al.
Nature, 508(7495), 254-257 (2014-02-18)
Zoonotic infectious diseases such as influenza continue to pose a grave threat to human health. However, the factors that mediate the emergence of RNA viruses such as influenza A virus (IAV) are still incompletely understood. Phylogenetic inference is crucial to reconstructing
Nenad Macesic et al.
The Medical journal of Australia, 198(7), 370-372 (2013-04-16)
To review cases of nosocomial influenza and compare the epidemiology, clinical characteristics and outcomes with community-acquired cases. Prospective case series of adults hospitalised with influenza during April - November of 2010 and 2011 using a hospital-based sentinel surveillance system. A

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