N3038

Anti-Nanog antibody, Mouse monoclonal

clone NNG-811, purified from hybridoma cell culture

• Synonym(s): Anti-NANOG/STM1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: NNG-811, monoclonal
• form: buffered aqueous solution
• mol wt: ~40 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 4-8 μg/mL
• isotype: IgG1
• UniProt accession no.: Q9H9S0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NANOG(79923)

Description

General description

Anti-Nanog antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma NNG-811 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human Nanog. The human Nanog gene product is a 305 amino acid, 35 kDa protein with a tryptophan repeat domain and two C-terminal trans-activating domains. It is primarily located in the nucleus.

Nanog is a homeodomain transcription factor expressed in cells of the inner cell mass of early embryos, in embryonic stem cells (ESC), germ cells and in stem/progenitor cells of some tissues. Together with Oct-4, Sox-2, and Rex-1, it is a molecular marker for pluripotent cells and for undifferentiated stem cells. This protein is crucial for the maintenance of pluripotency of ESCs and is down-regulated when ESCs differentiate.
Nanog controls the expression of many ESC genes together with other stem cell transcription factors like Oct-4 and Sox-2. Nanog targets both repressor and activator complexes to regulatory regions of hundreds of genes in the genome. Expression of nanog can be detected primarily in germ cell tumors and in tumors of other cell types. Nanog is an important marker for Seminomas, testicular carcinomas, teratocarcinomas, and germ cell-like tumors in various tissues. Furthermore, it was shown to transform NIH3T3 cells.

Specificity

Monoclonal Anti-Nanog reacts specifically with human Nanog.

Immunogen

recombinant human Nanog.

Application

Anti-Nanog antibody, Mouse monoclonal has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• flow cytometric analysis
• immunoprecipitation
• immunofluorescence

Monoclonal Anti-Nanog antibody is suitable for use in ELISA, immunoblotting (~ 40 kDa), immunocytochemistry and immunoprecipitation.
Immunoblotting: a working antibody concentration of 2-4 mg/mL is recommended using extracts of NT2 cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves