Each well was seeded with 10,000 cells in 10% FBS DMEM for 18 hours. Subsequently, the media was replaced with 1% FBS DMEM containing up to 1 M 2-Deoxy-D-Glucose. After 24 hours, the cell media was removed for the assay. Typically, each well is seeded with 100 µL of 10,000 cells.
MAK439
Glycolysis Assay Kit
Sufficient for 100 colorimetric tests
Synonym(s):
Cellular Glycolysis Detection Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
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Quality Level
detection method
colorimetric
relevant disease(s)
diabetes; cancer; endocrinological disorders
storage temp.
−20°C
Related Categories
General description
Glycolysis is one of the major metabolic pathways cells undergo to produce energy and results in the production of pyruvate. One of the eventual end products of this process is lactate dehydrogenase-mediated conversion of pyruvate to L-lactate via lactic acid fermentation. This enzymatic mechanism allows L-lactate to serve as an indicator of glycolysis.
Application
The Glycolysis Assay Kit may be used for:
- Cancer Research
- Diabetes Research
- Cardiovascular Disease Research
Features and Benefits
Broad Detection Range: Detect L-lactate levels accurately up to 10 mM, using only 5 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Suitability
The kit is suitable for the direct determination of L-Lactate produced by glycolysis in cell media samples and for the screening of glycolysis inhibitors.
Principle
The Glycolysis Assay Kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH, which subsequently reduces a formazan dye. The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to the glycolytic rate of the cells.
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
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In the MAK439-1KT Glycolysis Assay Kit PANC1 cells in DMEM medium were treated with varying concentrations of 2-deoxy-D-glucose to inhibit glycolysis. please provide details on the number of cells initially seeded and in what volume, as well as the duration for which the PANC1 cells were treated with 2DG before samples were taken for the glycolysis assay?
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