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MAK076

Sigma-Aldrich

Ethanol Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

The new Ethanol Assay Kit, MAK481, is now available! Ethanol is a psychoactive component of many commonly consumed drinks where it acts as a central nervous system depressant. After ingestion, ethanol is absorbed into the bloodstream via the stomach and small intestine. Ethanol is largely metabolized by the liver but is also secreted in urine or through respiration. The monitoring of ethanol levels is also important in fermentation processes. The ethanol assay kit provides a simple and reliable method for the quantification of ethanol.
This kit is highly sensitive to the presence of short-chain alcohols (ethanol, methanol, and propanol). Storage or use of this kit in the vicinity of alcohol vapors can result in the uptake of the alcohols by kit components, resulting in very high backgrounds.

Application

Ethanol Assay Kit has been used :
  • to measure the levels of ethanol in yeast cell culture supernatant and to study the link between metabolism and chromatin structure[1]
  • to determine the ethanol levels in compound beverages and foods[2]
  • to measure ethanol content in vinegar[3]

Suitability

Suitable for determining the concentration of ethanol in serum, plasma, and other body fluids as well as in beverages and growth media.

Principle

Ethanol concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λex = 587 nm) product, proportional to the ethanol present. The Ethanol Assay Kit provides a simple and reliable method for the quantification of ethanol in serum, plasma, and other body fluids as well as in beverages and growth media.

replaced by

Product No.
Description
Pricing

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8M02K06200
7K18K06200
8D14K06200
8H02K06200
7E19K06200

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Detecting ethanol and acetaldehyde by simple and ultrasensitive fluorimetric methods in compound foods.
Zachut M, et al.
Food Chemistry, 201, 270-274 (2016)
Metabolite profiling and volatiles of pineapple wine and vinegar obtained from pineapple waste.
Roda A, et al.
Food Chemistry, 229, 734-742 (2017)
Michael S Patton et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 46(2), 351-359 (2020-07-15)
Compulsive alcohol consumption is a core, treatment-resistant feature of alcohol use disorder. The dorsomedial and dorsolateral striatum support goal-directed and habitual action strategies, respectively. How ethanol targets dorsolateral striatum to drive compulsive consumption is poorly understood. Parvalbumin-expressing striatal fast-spiking interneurons
Shiyu Xu et al.
eNeuro, 5(3) (2018-06-19)
Ethanol has robust effects on presynaptic activity in many neurons, however, it is not yet clear how this drug acts within this compartment to change neural activity, nor the significance of this change on behavior and physiology in vivo. One
Francisca Vaz Bravo et al.
Scientific reports, 8(1), 3540-3540 (2018-02-25)
Phospholipase D (PLD) is a key player in the modulation of multiple aspects of cell physiology and has been proposed as a therapeutic target for Alzheimer's disease (AD). Here, we characterize a PLD mutant, pld-1, using the Caenorhabditis elegans animal
View All Related Papers

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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