M4278

Monoclonal Anti-MAP1 antibody produced in mouse

clone HM-1, ascites fluid

• Synonym(s): Anti-MAP1a
• MDL number: MFCD00164681
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: HM-1, monoclonal
• contains: 15 mM sodium azide
• species reactivity: mouse, rat
• technique(s): immunohistochemistry (frozen sections): suitable; microarray: suitable; western blot: 1:500 using a fresh total rat brain extract or an enriched microtubule protein preparation
• isotype: IgG1
• UniProt accession no.: Q9QYR6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Mtap1a(17754) ; rat ... Map1a(25152)

Description

General description

Monoclonal Anti-MAP1 (mouse IgG1 isotype) is derived from the HM-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a rat brain MAPs preparation. MAP1 is one of the major neuronal MAPs as well as being the largest (350 kD). MAP1 is more generally distributed, being found in both dendrites and axons of neurons and in glial cells in brain, in chromatophores and on both interphase and mitotic microtubules in various tissue culture cells.

Specificity

The antibody does not cross-react with other MAPs or tubulin. By immunohistochemical staining of brain tissue, the antibody shows selective labeling of neurons with stronger staining of axons than dendrites.

Immunogen

rat brain microtubule-associated proteins (MAPs)

Application

Monoclonal Anti-MAP1 has been used in :

• immunoblotting
• dot blot
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions

Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins (MAPs). MAPs are known to mediate the binding of membranous organelles, actin filaments and intermediate filaments to microtubules. Therefore, it might be important for cellular processes such as mitosis and organelle transport, and for determining the dynamic properties of the cytoskeleton.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1