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H6914

Sigma-Aldrich

Human Serum

(from male AB clotted whole blood), USA origin, sterile-filtered

Synonym(s):

human sera, human serum, off the clot, otc

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About This Item

MDL number:
MFCD00165829
UNSPSC Code:
12352207
NACRES:
NA.71
biological source:
human
origin:
USA origin
sterility:
sterile-filtered
shipped in:
dry ice
Pricing and availability is not currently available.

biological source

human

Quality Level

300

sterility

sterile-filtered

composition

Hemoglobin, ≤25 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

impurities

≤10 EU/mL Endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Human AB serum is used in tissue engineering, transplantation and cell therapy applications for the expansion of mesenchymal stem cells (MSC) from adipose tissue or mesenchymal stromal cells from human bone marrow; for standardized limbal epithelial stem cell graft generation and transplantation; for ex vivo expansion of NK cells from peripheral blood in Hematopoeitic Stem Cell Expansion Medium; and for upgrading pretransplant human islet culture technology.

Packaging

Packaging may vary for different batches due to short supply of the bottles used to pack this product. The presence or lack of shrink wrap around the cap of the bottle doesn’t impact product integrity/sterility of the product.

Warning

Each donor is tested for and found non-reactive for Hepatitis B & C and non-reactive for Human Immunodeficiency Virus (HIV) antibody by ELISA. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly.

Analysis Note

Endotoxin tested

Other Notes

It is not unusual for our human serum products to be cloudy. This product is from pooled serum and cloudiness indicates that lipids are present in the serum. No clear, lipid-free human serum products are available.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000298253
0000298253
0000298252
0000298252
0000343425

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Ankit Sinha et al.
Cell systems, 8(4), 345-351 (2019-04-15)
High-grade serous ovarian carcinoma (HGSC) is the most common and lethal subtype of gynecologic malignancy in women. The current standard of treatment combines cytoreductive surgery and chemotherapy. Despite the efficacy of initial treatment, most patients develop cancer recurrence, and 70%
Kwang Hoe Kim et al.
Analytica chimica acta, 882, 38-48 (2015-06-05)
Multiple reaction monitoring (MRM) is commonly used for the quantitative analysis of proteins during mass pectrometry (MS), and has excellent specificity and sensitivity for an analyte in a complex sample. In this study, a pseudo-MRM method for the quantitative analysis
Alejandro Bustamante et al.
Stroke, 48(9), 2419-2425 (2017-07-19)
Stroke diagnosis could be challenging in the acute phase. We aimed to develop a blood-based diagnostic tool to differentiate between real strokes and stroke mimics and between ischemic and hemorrhagic strokes in the hyperacute phase. The Stroke-Chip was a prospective
Laura Abraira et al.
Epilepsia, 61(10), 2244-2253 (2020-08-29)
Blood biomarkers have not been widely investigated in poststroke epilepsy. In this study, we aimed to describe clinical factors and biomarkers present during acute stroke and analyze their association with the development of epilepsy at long term. A panel of
Hien T Ngoc Le et al.
Micromachines, 11(9) (2020-08-23)
Amyloid beta (aβ) 1-42, a peptide that is 1-42 amino acids long, is a major component of senile plaques in the brains of patients with Alzheimer's disease. Aβ detection has become an essential antecedence to predict the declining mental abilities
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Questions

1–9 of 9 Questions  

  1. What is the best way to thaw this product? Once thawed, how long can we use it if kept in at 4 oC?

    1 answer

    1. The recommended storage temperature is -20°C. The following steps confirm the best way to thaw serum:

      1. Remove the serum bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
      2. Place each container in a 30 to 37 °C water bath or incubator. Excessive temperatures will degrade heat-labile nutrients. If using a
      water bath, prevent the bottle caps from being completely submerged.
      3. Gently swirl or shake the bottles every 10 - 15 minutes until the serum is completely thawed.
      Please note: Multiple thaw/freeze cycles should be avoided as they will hasten serum nutrient degradation and can induce insoluble precipitate formation.

      Helpful?

  2. Is this serum manufactured under cGMP?

    1 answer

    1. This product is not manufactured under cGMP.

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  3. If the serum is being used to complete media for a cell thaw but then the cells are put through a DNA extraction rather than put into culture - would thawing a bottle of serum, aliquoting, and then re-freezing the serum be okay?

    1 answer

    1. Thawed serum should be mixed thoroughly before being added to cell culture medium. Repeated freeze-thaw cycles may significantly impact serum quality. It is recommended to aliquot the thawed serum for single use and store aliquots at -20 °C. If serum is stored between 2-8 °C, it should be used within 2-4 weeks.

      Helpful?

  4. DOES THIS PRODUCT CONTAIN OESTRADIOL

    1 answer

    1. The presence of oestradiol in this product is not determined. To see which compounds are tested for, refer to a sample Certificate of Analysis found at: https://www.sigmaaldrich.com/product/sigma/h6914#product-documentation

      Helpful?

  5. At which biosafety level should Human Serum H6914 be handled?

    1 answer

    1. Blood products should be treated and handled as potentially infectious. It is recommended to use human origin products from normal (non-diseased) donors at Biosafety Level 2 (BSL-2). Although donors may be tested for adventitious infections, as listed on the Certificate of Analysis for the product, no testing can cover all types of infection. Therefore, these products should be treated as potentially infectious and handled accordingly. Laboratory directors and principal investigators are responsible for performing a risk assessment and determining the biosafety level in accordance with applicable national and local regulations.

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  6. Human Serum (Product No. H6914 & Batch No. SLCF0424)의 사용기한은 CoA상에서 2023년 2월 까지인 것으로 확인됩니다. 혹시 해당 batch의 사용기한이 연장될 수도 있을까요?

    1 answer

    1. The shelf life is not extended for those product which are assigned a firm expiration date. No further testing is performed on these lots.

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  7. Are all your human donors consented?

    1 answer

    1. All protocols including donor consent are subject to the strict oversight of an Institutional Review Board (IRB) as governed by US law.

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  8. What is the difference between human serum from AB plasma and  human serum from AB clotted whole blood?

    1 answer

    1. Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present.  The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma

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  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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