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G0792

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor from rat

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophililzed powder

Synonym(s):

GM-CSF

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Granulocyte-Macrophage Colony-Stimulating Factor from rat, GM-CSF, recombinant, expressed in E. coli

biological source

rat

Quality Level

recombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.1 ng/mL

quality

endotoxin tested

mol wt

14.7 kDa

packaging

pkg of 5X5 μg
pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

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Biochem/physiol Actions

Four distinct colony-stimulating factors (CSFs) promoting survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte-macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), and interleukin-3 (IL-3, Multi-CSF). GM-CSF is a multipotential growth factor, stimulating proliferation of progenitor cells from more than one hematopoietic lineage (granulocyte, macrophage, and eosinophil). GM-CSF stimulates colony formation from pluripotent progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. GM-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. It is produced by various cell types (activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to cytokine and inflammatory stimuli. In addition to granulocyte-macrophage progenitors, GMCSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF can induce nonhematopoietic cells such as endothelial cells to migrate and proliferate. It also stimulates the proliferation of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity GM-CSF receptors contain a GM-CSF-specific α?chain and a common α ?chain.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

Preparation Note

Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced from a DNA sequence encoding mature rat GM-CSF. This recombinant protein is a mixture of two rat GM-CSFs, the 127 amino acid form and the 128 amino acid methionyl form. Mature rat GM-CSF has a calculated mass of 14.7 kDa. GM-CSF, an acidic glycoprotein, is species-specific.

Analysis Note

The biological activity is measured by its ability to induce proliferation of the factor-dependent mouse cell line, DA3.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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