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D0443

Sigma-Aldrich

Dextranase from Chaetomium erraticum

Synonym(s):

Dextranase Plus L, 1,6-α-D-Glucan 6-glucanohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

solution

storage temp.

2-8°C

General description

A product of Novozymes Corp.
Dextranase is generated by many microorganisms, including bacteria, filamentous fungi, and yeast.

Application

Dextranase from Chaetomium erraticum has been used:
  • in microcarrier dissolution to produce multicellular 3D spheroids for drug discovery
  • in in vitro digestion of extracellular polysaccharide (EPS) from the bacterial isolates
  • in a study to investigate the immobilization of dextranase
  • to investigate the optimization of process conditions for enzymatic modification of alternan

Biochem/physiol Actions

Dextranase is an endodextranase that hydrolyzes α-(1,6)-glycosidic linkages in dextran. It has many industrial and clinical applications such as food processing, sugar modification, and medicine preparation. Dextranase is widely used to produce alternated sugar beet and isomaltooligosaccharides (IMOs), a prebiotic functional food. The low molecular weight dextran obtained from dextranase serves as a synthetic blood volume expander. Dextranase can be coupled to antibodies for the detection of tumor cells. It prevents the occurrence of dental plaques by depolymerizing microbial dextran deposits in teeth. Hence, it is used in dental care products.

Preparation Note

A fungal dextranase produced by submerged fermentation of Chaetomium erraticum.

Analysis Note

Stable in the pH range of 3-7 and at temperatures up to approx. 70 °C. For most applications, the preferred conditions are pH 5-6 and a temperature of 50-60 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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