1321N1 Cell Line human

86030402, human brain (astrocytoma), Glial

• Synonym(s): 1321-N1 Cells
• UNSPSC Code: 41106514

Properties

• Product Name: 1321N1 Cell Line human, from human brain(astrocytoma), 86030402
• biological source: human brain (astrocytoma)
• growth mode: Adherent
• karyotype: Not specified
• morphology: Glial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human brain astrocytoma

Cell Line Description

1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten .1321N1 has been shown to have very similar STR profile data to U-118 MG.

Application

Human 1321N1 Cell Line has been used to study how the multiscale morphology of functional thin films affects the in vitro behavior of human neural astrocytoma 1321N1 cells.[1] It has also been used to study the expression and reactivity of Caytaxin protein.[2]

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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