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404R-1

Sigma-Aldrich

C4d (SP91) Rabbit Monoclonal Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

SP91, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (404R-14)
vial of 0.5 mL concentrate (404R-15)
bottle of 1.0 mL predilute (404R-17)
vial of 1.0 mL concentrate (404R-16)
bottle of 7.0 mL predilute (404R-18)

manufacturer/tradename

Cell Marque®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200

isotype

IgG

control

lymph node, tonsil

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic, membranous

Gene Information

human ... C4B(721)

Related Categories

General description

Acute humoral rejection is mediated by antibodies to the donor endothelium that activates the classical complement pathway.[1][2] This leads to a number of split products of complement proteins. C4d is a fragment of C4 complement released during activation of the classic complement pathway by the antigen-antibody complex.[3] C4d deposits in peritubular capillaries and is regarded as an indirect sign of an antibody response.[3][4] C4d can be a useful tool for indicating acute renal allograft rejection.

Quality


IVD
IVD
IVD
RUO

Linkage

C4d Positive Control Slides, Product No. 404S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: [email protected]

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000414915
0000414915
0000404801
0000404801
0000404795

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Chen Jianghua et al.
Clinical transplantation, 19(6), 785-791 (2005-11-30)
To determine the diagnostic and clinical significance of C4d accumulation in renal allografts followed by acute rejection. A total of 158 graft biopsies performed from December 1997 to December 2002 were classified, according to the Banff-97 criteria, into hyperacute rejection
Christian A Seemayer et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 22(2), 568-576 (2006-12-14)
Detection of C4d along peritubular capillaries (PTC) in renal allograft biopsies is an independent prognostic marker of poor long-term graft survival. It is typically associated with circulating donor-specific antibodies. Since only little information is available on the best technique to
Gyongyi M Nadasdy et al.
Human pathology, 36(11), 1178-1185 (2005-11-02)
Detection of peritubular capillary (PTC) C4d deposition in tissue sections of renal allograft biopsies became an important aid in the diagnosis of antibody-mediated rejection. Pathologists in many major transplant centers now routinely stain renal allograft biopsies for C4d. Currently, there
Dorothée Bouron-Dal Soglio et al.
Human pathology, 39(7), 1103-1110 (2008-06-24)
C4d is a marker of the activated complement cascade used to assess the humoral component of rejection, mostly in kidney allograft transplants. The role of C4d deposition has recently been addressed in hepatic allograft but has never been tested in
Liise K Kayler et al.
Transplantation, 85(6), 813-820 (2008-03-25)
Focal PTC C4d staining in acute renal allograft rejection has not been studied extensively. Renal allograft biopsies performed after October 2003, representing the first episode of acute rejection (AR) in recipients with > or = 12 months follow-up postbiopsy, were
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Questions

  1. How to detect kidney-related diseases and is interested in using C4d. What other antibodies can be used with this antibody? Can 404A be used for IF?

    1 answer

    1. C3d is typically a companion product for transplant rejection. For a kidney panel in general, the following markers are available:

      - Renal Epithelial Tumors: RCC, CD10, Pax2, Vimentin, Ksp-Cadherin, Parvalbumin, CD117, TFE3, Ep-CAM, Caveolin-1
      - Clear cell RCC: + + + + - - - + - -
      - Chromophobe: - - - - + + + + + +
      - Oncocytoma: - - - - + + + - - -

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