Skip to Content
Merck
All Photos(1)

Documents

19362

Sigma-Aldrich

4-Methylumbelliferyl butyrate

lipase substrate; esterase substrate, ≥95% (HPCE), powder or crystals, suitable for fluorescence

Synonym(s):

MU-Bu

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C14H14O4
CAS Number:
17695-46-4
Molecular Weight:
246.26
Beilstein:
227973
EC Number:
241-694-7
MDL number:
MFCD00038087
UNSPSC Code:
12352204
PubChem Substance ID:
57647789
NACRES:
NA.32

product name

4-Methylumbelliferyl butyrate, suitable for fluorescence, ≥95% (HPCE)

Quality Level

100

Assay

≥95% (HPCE)

form

powder or crystals

mp

90-92 °C (lit.)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 312 nm in methanol
λex 360 nm; λem 449 nm (Reaction product)
λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3

InChI key

WKPUJZVCZXWKCK-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

suitable as fluorogenic substrate for esterases/lipases

Other Notes

Fluorogenic substrate for butyrate esterase

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Determination of lipase activity using image analysis.
Marcelo Müller-Santos et al.
Analytical biochemistry, 351(2), 305-307 (2006-03-10)
M Vaneechoutte et al.
Journal of clinical microbiology, 26(6), 1227-1228 (1988-06-01)
Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5
Ivan Kurtovic et al.
Fish physiology and biochemistry, 36(4), 1041-1060 (2010-02-10)
Lipases were purified from delipidated pyloric ceca powder of two New Zealand-sourced fish, Chinook salmon (Oncorhynchus tshawytscha) and hoki (Macruronus novaezelandiae), by fractional precipitation with polyethylene glycol 1000, followed by affinity chromatography using cholate-Affi-Gel 102, and gel filtration on Sephacryl
Neda Gilannejad et al.
Animals : an open access journal from MDPI, 10(5) (2020-05-24)
Thick-lipped grey mullet (Chelon labrosus) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of
E Speeleveld et al.
Journal of clinical microbiology, 32(5), 1362-1363 (1994-05-01)
Moraxella catarrhalis can easily be differentiated from other oxidase-positive, gram-negative cocci with tributyrine, 4-methylumbelliferyl butyrate, or indoxyl acetate. All M. catarrhalis give positive reactions, and all Neisseria spp. give negative reactions. The 4-methylumbelliferyl butyrate tube test and indoxyl acetate strip
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service