If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
M9929
Methotrexate
meets USP testing specifications
Synonym(s):
Methotrexate hydrate, 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate
About This Item
Recommended Products
biological source
synthetic
Quality Level
Agency
USP/NF
meets USP testing specifications
Assay
98.0-102.0%
form
powder
storage condition
(Tightly closed. Dry. Keep in a well-ventilated place. )
technique(s)
activity assay: suitable
impurities
≤0.1% Residue on ignition (Ash)
≤12.0% water (Karl Fischer)
color
yellow to orange-yellow
mp
185-204 °C (365 - 399 °F)
solubility
water: insoluble
antibiotic activity spectrum
parasites
application(s)
metabolomics
microbiology
Mode of action
DNA synthesis
storage temp.
−20°C
SMILES string
[H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
InChI
1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
InChI key
FPJYMUQSRFJSEW-ZOWNYOTGSA-N
Gene Information
human ... DHFR(1719) , DHFRP1(573971) , FPGS(2356) , SLC19A1(6573) , TYMS(7298)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)
Looking for similar products? Visit Product Comparison Guide
Application
Biochem/physiol Actions
Other Notes
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
Cancer research innovations address the complexity of the disease, providing advanced technologies for varied applications.
Cancer research innovations address the complexity of the disease, providing advanced technologies for varied applications.
Biofiles reviews innovative technologies for cancer research, reflecting the complexity of the disease.
Cancer research innovations address the complexity of the disease, providing advanced technologies for varied applications.
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
To prepare methotrexate stock solution, should methotrexate, product M9929, be dissolved in 0.1 M sodium hydroxide or 1 M?
1 answer-
Methotrexate is typically dissolved in a 0.1 M sodium hydroxide solution to prepare a stock solution. This concentration is sufficient to ensure complete dissolution, as methotrexate is slightly soluble in water. Using a 1 M sodium hydroxide solution may be too strong and could potentially alter the stability of methotrexate.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Can Product M9929, Methotrexate, be stored?
1 answer-
Alkaline stock solutions can decompose. It is best to prepare stock solutions in a minimal amount of of 1 M sodium hydroxide, then dilute with saline or medium. Solutions can be stored at -20°C for about one month. Solutions can be stored for about 1 week at 2-8°C.
Helpful?
-
-
What is the solubility of Methotrexate, Product M9929?
1 answer-
Methotrexate is practically insoluble in water. It does dissolve in solutions of mineral acids (such as HCl) and in dilute solutions of alkali hydroxides. A solution can be prepared in 0.1 M sodium hydroxide at 20 mg/ml (may require some heating).
Helpful?
-
-
How can I sterilize Product M9929, Methotrexate solutions?
1 answer-
Solutions can be sterile filtered.
Helpful?
-
-
Has Product M9929, Methotrexate, been tested for cell culture applications?
1 answer-
Product M9929 has not been cell culture tested. Product M8407, Methotrexate hydrate, has been both mammalian and insect cell culture tested and is recommended to use for these applications.
Helpful?
-
-
What does the description of "meets USP testing specification" signify?
1 answer-
The product has been tested according to the specifications in the monograph of the United States Pharmacopoeia.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service