Alkaline stock solutions can decompose. It is best to prepare stock solutions in a minimal amount of of 1 M sodium hydroxide, then dilute with saline or medium. Solutions can be stored at -20°C for about one month. Solutions can be stored for about 1 week at 2-8°C.
M9929
Methotrexate
meets USP testing specifications
Synonym(s):
Methotrexate hydrate, 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate
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All Photos(4)
About This Item
Empirical Formula (Hill Notation):
C20H22N8O5 · xH2O
CAS Number:
Molecular Weight:
454.44 (anhydrous basis)
MDL number:
UNSPSC Code:
12352005
PubChem Substance ID:
NACRES:
NA.21
Pricing and availability is not currently available.
Recommended Products
biological source
synthetic
Quality Level
Agency
USP/NF
meets USP testing specifications
Assay
98.0-102.0%
form
powder
storage condition
(Tightly closed. Dry. Keep in a well-ventilated place. )
technique(s)
activity assay: suitable
impurities
≤0.1% Residue on ignition (Ash)
≤12.0% water (Karl Fischer)
color
yellow to orange-yellow
mp
185-204 °C (365 - 399 °F)
solubility
water: insoluble
antibiotic activity spectrum
parasites
application(s)
metabolomics
microbiology
Mode of action
DNA synthesis
storage temp.
−20°C
SMILES string
[H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
InChI
1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
InChI key
FPJYMUQSRFJSEW-ZOWNYOTGSA-N
Gene Information
human ... DHFR(1719) , DHFRP1(573971) , FPGS(2356) , SLC19A1(6573) , TYMS(7298)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)
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Application
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Biochem/physiol Actions
Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.
Other Notes
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Can Product M9929, Methotrexate, be stored?
1 answer-
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Has Product M9929, Methotrexate, been tested for cell culture applications?
1 answer-
Product M9929 has not been cell culture tested. Product M8407, Methotrexate hydrate, has been both mammalian and insect cell culture tested and is recommended to use for these applications.
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How can I sterilize Product M9929, Methotrexate solutions?
1 answer-
Solutions can be sterile filtered.
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What is the solubility of Methotrexate, Product M9929?
1 answer-
Methotrexate is practically insoluble in water. It does dissolve in solutions of mineral acids (such as HCl) and in dilute solutions of alkali hydroxides. A solution can be prepared in 0.1 M sodium hydroxide at 20 mg/ml (may require some heating).
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What does the description of "meets USP testing specification" signify?
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The product has been tested according to the specifications in the monograph of the United States Pharmacopoeia.
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