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556416

Sigma-Aldrich

New Methylene Blue N

Dye content ~70 %

Synonym(s):

Basic Blue 24, Methylene Blue N

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About This Item

Empirical Formula (Hill Notation):
C18H22ClN3S
CAS Number:
1934-16-3
Molecular Weight:
347.91
Colour Index Number:
52030
EC Number:
217-696-9
MDL number:
MFCD00135878
UNSPSC Code:
12171500
PubChem Substance ID:
24879556
NACRES:
NA.47
Pricing and availability is not currently available.

form

powder, crystals or chunks

composition

Dye content, ~70%

technique(s)

microbe id | staining: suitable

mp

190 °C (dec.) (lit.)

λmax

590 nm
632 nm (2nd)

εmax

≥29000 at 626-634 nm in water
≥30500 at 586-594 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CCNc1cc2[s+]c3cc(NCC)c(C)cc3nc2cc1C

InChI

1S/C18H22N3S.ClH/c1-5-19-13-9-17-15(7-11(13)3)21-16-8-12(4)14(20-6-2)10-18(16)22-17;/h7-10,19-20H,5-6H2,1-4H3;1H/q+1;/p-1

InChI key

NZYCYASKVWSANA-UHFFFAOYSA-M

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Application

New methylene blue N has been used as a photosensitizer for the photodynamic inactivation of Candida albicans.[1][2]

Biochem/physiol Actions

New methylene blue N can bind, neutralize and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N helps in counting of reticulocytes (erythrocytes containing RNA).[3]

Features and Benefits

Solvent soluble cationic thiazine dye

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000376807
0000376807
0000164445
0000164445
SHBN9708

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Fernando A Castro et al.
Veterinary surgery : VS, 34(6), 539-542 (2005-12-14)
To evaluate the accuracy of a new technique for perineural injection of the lateral palmar nerve and to determine frequency of inadvertent injection into the carpal synovial sheath with this technique. Prospective experimental study. Thirty equine cadaver forelimbs. Each of
M Wainwright et al.
Journal of chemotherapy (Florence, Italy), 13(5), 503-509 (2002-01-05)
The antibacterial activities of Methylene Blue and several of its congeners were measured against Yersinia enterocolitica, a gram-negative pathogen known to exhibit significant growth at 4 degrees C and thus constituting a threat to red blood cell concentrates which are
Puja Paul et al.
Journal of photochemistry and photobiology. B, Biology, 204, 111804-111804 (2020-02-03)
The ubiquitous influence of double stranded RNAs in biological events makes them imperative to gather data based on specific binding procedure of small molecules to various RNA conformations. Particular interest may be attributed to situations wherein small molecules target RNAs
Turgeon ML
Linne & Ringsrud's Clinical Laboratory Science: The Basics and Routine Techniques (2014)
Tianhong Dai et al.
Antimicrobial agents and chemotherapy, 55(12), 5710-5717 (2011-09-21)
The objective of this study was to investigate photodynamic therapy (PDT), using blue dye and red light, for prophylaxis and treatment of cutaneous Candida albicans infections in mice. A mouse model of skin abrasion infected with C. albicans was developed
View All Related Papers

Questions

  1. I would like to use this to stain blood smears to identify/count reticulocytes. What volume of stain would 1g of the powder make?

    1 answer

    1. Instructions for use are not available for this product. However, product R4132 is a prepared solution specifically for use in reticulocyte staining. The New Methylene Blue concentration of this solution is 1% or 1g/100 mL The product formulation is as follows:
      New Methylene blue, 1%
      Sodium Chloride, 0.9%
      Potassium Oxalate, 1.6%

      Based on the formulation listed above, the end-user will be able to prepare 100 ml of a 1% solution if using product 556416. This would also require the addition of the sodium chloride and potassium oxalate components.
      Please see the link below to review the product datasheet for the prepared stain R4132:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/236/942/procedure-r4132-reticulocytestain-ms.pdf

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