10775835001
Roche
Bovine Serum Albumin
98.5% (electrophoresis), Fatty acids (total) ≤0.2 mg/g, Triglycerides (enzym.) free, Immunoglobulins not detectable, microbiological culture: suitable, USA origin
Synonym(s):
BSA, Bovine Serum Albumin Fraction V, fatty acid free, Bovine albumin, bovine serum
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About This Item
UNSPSC Code:
12352106
Recommended Products
biological source
bovine
Quality Level
Assay
98.5% (electrophoresis)
form
lyophilized
mol wt
Mr 68 kDa
packaging
pkg of 50 g
manufacturer/tradename
Roche
origin
USA origin
technique(s)
immunofluorescence: suitable
ligand binding assay: suitable
impurities
Immunoglobulins, none detected
Triglycerides, none detected
≤0.2 mg/g fatty acids
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.
Application
Bovine Serum Albumin, Fraction V, fatty acid free is used:
- in lipid research to study lipid metabolism in biological systems
- to identify transport mechanisms in living organisms
- to study ligand binding
- to investigate ionic binding
- in the preparation of solutions during isolation of cardiomyocytes
- as a blocking solution to incubate embryos for immunofluorescence
- in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers
Biochem/physiol Actions
The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.
Features and Benefits
- Free from fatty acids according to current quality control procedures (suitable for lipid research)
- High quality
- Low content of heavy metal ions
- Long-term stability
Quality
Purity: ≥98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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