This product is supplied in a vial containing 250mg of lyophilized power. The activity is not determined. The C1-BIOC is also a powder form. The minimum activity is 125 units per milligram. For high activity, highly purified products, see C0773 and C2399. A link to each product page is below:
https://www.sigmaaldrich.com/product/sigma/c0773
https://www.sigmaaldrich.com/product/sigma/c2399
SCR103
Collagenase type I
The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.
Synonym(s):
Collagenase Type I from Clostridium histolyticum, Tissue dissociation
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About This Item
UNSPSC Code:
12352204
eCl@ss:
32160801
NACRES:
NA.75
Pricing and availability is not currently available.
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Quality Level
form
powder
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
shipped in
wet ice
General description
Collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.
Packaging
1 vial containing 250mg of lyophilized powder
Quality
Each lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore′s HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.
Physical form
Collagenase type I is supplied as a dialyzed, lyophilized powder.
Storage and Stability
Collagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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안녕하세요. 제품관련 문의드립니다. 이 제품 속성이 liquid로 되어있고 포장형태는 파우더라고 되어있는데.. 어떤형태로 제공되나요? 몇 UI 짜리인가요? 동물 힘줄에 injection 할 건데.. 좀더 큰 용량의 다른 제품은 없나요? 함께 검색되는 C1-BIOC(100 mg과 1g) 짜리 제품을 사용해도 되나요? 궁금합니다.
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