ABN2266
Anti-Tentonin 3/TMEM150c
from rabbit
Synonym(s):
Transmembrane protein 150C, TTN3, Tentonin 3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
mouse ... Tmem150C(231503)
General description
Transmembrane protein 150C (UniProt: Q8C8S3; also known as Tentonin, TTN3) is encoded by the Tmem150c gene (Gene ID: 231503) in murine species. TTN3 is a multi-pass membrane protein of the DRAM/TMEM150 family. Two isoforms of TTN3 have been described that are produced by alternative splicing. It belongs to a new class of mechanotransduction channel that is expressed in muscle spindle afferents and contribute to proprioception (touch sensation). It is activated by mechanical stimuli with distinctly slow inactivation kinetics. Mice with deficiency in TTN3 display loss of coordinated movements and abnormal gait. It is reported to mediate slow adapting currents in DRG neurons.
Specificity
This rabbit polyclonal antibody detects Transmembrane protein 150C in mouse and rat cells. it targets an epitope within 38 amino acids from the C-terminal region.
Immunogen
A linear peptide corresponding to 38 amino acids from the C-terminal region of murine Transmembrane protein 150C.
Epitope: C-terminus
Application
Anti-Tentonin 3/TMEM150c, Cat. No. ABN2266, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 150C and has been tested in , Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected Tentonin 3/TMEM150c in muscle spindle from mouse (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).
Immunocytochemistry Analysis: A representative lot detected Tentonin 3/TMEM150c in HEK293T cells transfected with TTN3. (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).
Western Blotting Analysis: A representative lot detected Tentonin 3/TMEM150c in F11 rat DRG cells (untransfected and TTN3 transfected cells) (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).
Immunocytochemistry Analysis: A representative lot detected Tentonin 3/TMEM150c in HEK293T cells transfected with TTN3. (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).
Western Blotting Analysis: A representative lot detected Tentonin 3/TMEM150c in F11 rat DRG cells (untransfected and TTN3 transfected cells) (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).
Research Category
Neuroscience
Neuroscience
Quality
Evaluated by Western Blotting in F11 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Tentonin 3/TMEM150c in 10 µg of F11 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Tentonin 3/TMEM150c in 10 µg of F11 cell lysate.
Target description
~25 kDa observed; 27.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Julia Ojeda-Alonso et al.
The Journal of general physiology, 154(12) (2022-10-19)
The membrane protein TMEM150C has been proposed to form a mechanosensitive ion channel that is required for normal proprioceptor function. Here, we examined whether expression of TMEM150C in neuroblastoma cells lacking Piezo1 is associated with the appearance of mechanosensitive currents.
Sungmin Pak et al.
Cell reports, 43(6), 114334-114334 (2024-06-08)
Mechanically activating (MA) channels transduce numerous physiological functions. Tentonin 3/TMEM150C (TTN3) confers MA currents with slow inactivation kinetics in somato- and barosensory neurons. However, questions were raised about its role as a Piezo1 regulator and its potential as a channel
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