AB3282
Anti-Glutathione-S-Transferase Antibody, S. japonicum form
Chemicon®, from rabbit
Synonym(s):
GST-tag
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
Schistosoma japonicum, vertebrates
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GSTP1(2950)
Specificity
Anti-Glutathione S-transferase (GST) is a polyclonal antibody to the recombinant glutathione S-transferase (GST) protein from Schistosoma japonicum.
The anti GST antibody reacts specifically with the glutathione S-transferase moiety of GST fusion proteins as expressed in E.coli by the pGEX (1) vectors and in yeast by the pYEX(TM) vectors (2).
(1)The pGEX expression vector system is a plasmid and purification system which, in most cases, results in the fusion protein being soluble in aqueous solutions for extraction and purification from crude bacterial lysates. This expression vector system enables the protein glutathione S-transferase (GST) to be produced in fusion with the protein of interest so as to bind with high affinity to a glutathione affinity column. The GST-protein complex can then be easily purified by elution from the column with reduced glutathione. The pGEX vectors are available as the GST System from Pharmacia Biotech.
(2) pYEX is a yeast GST expression vector supplied by AMRAD biotech of Australia.
The anti GST antibody reacts specifically with the glutathione S-transferase moiety of GST fusion proteins as expressed in E.coli by the pGEX (1) vectors and in yeast by the pYEX(TM) vectors (2).
(1)The pGEX expression vector system is a plasmid and purification system which, in most cases, results in the fusion protein being soluble in aqueous solutions for extraction and purification from crude bacterial lysates. This expression vector system enables the protein glutathione S-transferase (GST) to be produced in fusion with the protein of interest so as to bind with high affinity to a glutathione affinity column. The GST-protein complex can then be easily purified by elution from the column with reduced glutathione. The pGEX vectors are available as the GST System from Pharmacia Biotech.
(2) pYEX is a yeast GST expression vector supplied by AMRAD biotech of Australia.
Immunogen
Epitope: S. japonicum form
Recombinant glutathione S-transferase (GST) protein from Schistosoma japonicum.
Application
Anti-Glutathione-S-Transferase Antibody, S. japonicum form is an antibody against Glutathione-S-Transferase for use in ELISA & WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
The anti-GST antibody can be used to detect GST fusion proteins in Western blot analysis and ELISA studies.
Physical form
Format: Purified
Liquid in PBS, pH 7.4, containing 0.02% Tween 20 and 0.02% sodium azide as a preservative.
Storage and Stability
The anti GST antibody is shipped liquid at 4°C. The anti GST antibody is stable for up to 6 months when stored at 2-8°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Role of phosphatidylinositol 4,5-bisphosphate in regulating EHD2 plasma membrane localization.
Simone, LC; Caplan, S; Naslavsky, N
Testing null
Jin-Ying Lu et al.
Methods in molecular biology (Clifton, N.J.), 981, 151-165 (2013-02-06)
Emergence of proteome microarray provides a versatile platform to globally explore biological functions of broad significance. In the past decade, researchers have successfully fabricated functional proteome microarrays by printing individually purified proteins at a high-throughput, proteome-wide scale on one single
Serum Profiling for Identification of Autoantibody Signatures in Diseases Using Protein Microarrays.
Shabarni Gupta et al.
Methods in molecular biology (Clifton, N.J.), 1619, 303-315 (2017-07-05)
Protein microarrays are platforms for studying protein-protein interactions and identifying disease-related self-antigens/autoantigens, which elicit an immune response in a high-throughput format. Protein arrays have been extensively used over the past two decades for several clinical applications. By using this platform
Shabarni Gupta et al.
Oncotarget, 8(35), 58443-58456 (2017-09-25)
Meningiomas are one of the most common tumors of the Central nervous system (CNS). This study aims to identify the autoantibody biomarkers in meningiomas using high-density human proteome arrays (~17,000 full-length recombinant human proteins). Screening of sera from 15 unaffected
Carolyn Nicole Brown et al.
iScience, 24(10), 103184-103184 (2021-10-21)
The Ca2+/Calmodulin-dependent protein kinase II (CaMKII) is a central regulator of synaptic plasticity and has been implicated in various neurological conditions, including schizophrenia. Here, we characterize six different CaMKIIα variants found in patients with schizophrenia. Only R396stop disrupted the 12-meric
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service