07-441
Anti-dimethyl-Histone H3 (Lys9) Antibody
Upstate®, from rabbit
Synonym(s):
H3K9me2, Histone H3 (di methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
Sign Into View Organizational & Contract Pricing
All Photos(5)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, Schizosaccharomyces pombe, rat, chicken
manufacturer/tradename
Upstate®
technique(s)
dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
dimethylation (Lys9)
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Specificity
Expected to cross react with mouse, chicken, rat and fission yeast, but not budding yeast. Broad species cross-reactivity expected.
Immunogen
KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human Histone H3.
Application
Dot Blot Analysis:
Specificity confirmed by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of Histone H3 in western blot analysis of HeLa acid extracts (Figure A, Lane 4).
Immunocytochemistry:
Reported by an independent laboratory.
Specificity confirmed by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of Histone H3 in western blot analysis of HeLa acid extracts (Figure A, Lane 4).
Immunocytochemistry:
Reported by an independent laboratory.
Use Anti-dimethyl-Histone H3 (Lys9) Antibody (Rabbit Polyclonal Antibody) validated in DB, ICC, PIA, WB to detect dimethyl-Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9).
Quality
Routinely evaluated by Western Blot on HeLa acid extract and recombinant Histone H3 (cat. # 14-411).
Western Blot Analysis:
1:500 dilution of this lot detected dimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.
Western Blot Analysis:
1:500 dilution of this lot detected dimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.
Target description
17 kDa
Physical form
Format: Purified
Storage and Stability
1 year at -20°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
HeLa whole cell lysate, U2OS cell lysate.
HeLa whole cell lysate, U2OS cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
UV-B promotes rapid nuclear translocation of the Arabidopsis UV-B specific signaling component UVR8 and activates its function in the nucleus.
Kaiserli, E; Jenkins, GI
Plant Cell null
Differential epigenetic regulation within an Arabidopsis retroposon family.
Rangwala, SH; Richards, EJ
Genetics null
ROR1/RPA2A, a putative replication protein A2, functions in epigenetic gene silencing and in regulation of meristem development in Arabidopsis.
Xia, R; Wang, J; Liu, C; Wang, Y; Wang, Y; Zhai, J; Liu, J; Hong, X; Cao, X; Zhu, JK; Gong, Z
Plant Cell null
Yu Wang et al.
EMBO reports, 8(1), 77-83 (2006-11-18)
TOUSLED-like kinases (TLKs) are highly conserved in plants and animals, but direct evidence linking TLKs and transcriptional gene silencing is lacking. We isolated two new alleles of TOUSLED (TSL). Mutations of TSL in ros1 reactivate the transcriptionally silent 35S-NPTII transgene
Polycomb-group proteins repress the floral activator AGL19 in the FLC-independent vernalization pathway.
Schonrock, N; Bouveret, R; Leroy, O; Borghi, L; Kohler, C; Gruissem, W; Hennig, L
Genes & Development null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service